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Analysis of expression for HsAIRK and PLK family genes, that regulate chromosome segregation in mitosis, in tumors

肿瘤中调节有丝分裂中染色体分离的 HsAIRK 和 PLK 家族基因的表达分析

基本信息

批准号：
14571188
负责人：
OSADA Shinji
金额：
$ 2.05万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

Aurora family are highly expressed during M phase, and have been suggested to regulate centrosome function, chromosome segregation, and cytokinesis. In addition, PLK family is also intimately involved in spindle formation and chromosome segregation during mitosis. The purpose of this study was to determine whether PLK or Aurora family are overexpressed in primary colorectal cancer specimens as compared with normal colon mucosa and assess its relation to other kinases as a potential new tumor marker.1.PLKIn the present study, immunohistochemical analyses were performed of PLK1 expression in 78 primary colorectal cancers as well as 15 normal colorectal specimens. Furthermore, we examined the relationship between other kinases, Aurora-A and Aurora-C, and PLK1 expression. In normal colon mucosa, some crypt cells showed weak positive staining for PLK1 in 13 out of 15 cases. Elevated expression of PLK1 was observed in 57 (73.1%) of the colorectal cancers, statistically significant associatio … More ns being evident with pT, pN and the Dukes' classification. Values for lesions with high and low PLK1 expression were 54.7±10.3% (mean±SD) and 45.9±11.9%. PLK1 was significantly associated with Aurora-A, but PLK1 staining was more diffuse and extensive than for Aurora-A or Aurora-C.2.AuroraIn the present study, immunohistochemical analyses were performed of HsAIRK1 and HsAIRK3 expression in 78 primary colorectal cancers and 36 colorectal adenomas as well as 15 normal colorectal specimens. Elevated expression of HsAIRK1 was observed in 53 (67.9%) of the colorectal cancers, and of HsAIRK3 in 40 (51.3%). Furthermore, colorectal adenomas showed high expression of HsAIRK1 and HsAIRK3 in 11 (30.6%) and 7 (19.4%) cases, respectively, thus being intermediate between colorectal cancers and normal colorectal mucosa. Interestingly, HsAIRK1 overexpression was significantly associated with p53 accumulation in colorectal cancers.Our results suggest overexpression of PLK1 or Aurora A might be of pathogenic, prognostic and proliferative importance, so that these mitosis-related genes might have potential as a new tumor marker for colorectal cancers. Less

Aurora家族在M期高度表达，并被认为调节中心体功能、染色体分离和胞质分裂。此外，PLK家族还与有丝分裂过程中纺锤体的形成和染色体分离密切相关。本研究的目的是确定PLK或Aurora家族是否在原发性结直肠癌组织中过表达，并评估其与其他激酶作为一种潜在的新的肿瘤标志物的关系。1. PLK在本研究中，PLK 1在78例原发性结直肠癌和15例正常结直肠标本中的表达进行了免疫组织化学分析。此外，我们还研究了其他激酶Aurora-A和Aurora-C与PLK 1表达之间的关系。在正常结肠粘膜中，13例有部分隐窝细胞呈PLK 1弱阳性染色。在57例（73.1%）结直肠癌中观察到PLK 1表达升高，统计学显著相关性为： ...更多信息 ns与pT、pN和Dukes分类明显相关。PLK 1高表达和低表达病变的数值分别为54.7±10.3%（平均值±SD）和45.9± 11.9%。Aurora-A和Aurora-C与PLK 1的表达有显著相关性，但PLK 1的表达较Aurora-A和Aurora-C更广泛。2. Aurora 78例原发性结直肠癌、36例结直肠腺瘤和15例正常结直肠组织中HsAIRK 1和HsAIRK 3的表达情况进行了免疫组化分析。在53例（67.9%）结直肠癌中观察到HsAIRK 1的高表达，40例（51.3%）HsAIRK 3的高表达。大肠腺瘤中HsAIRK 1和HsAIRK 3的高表达分别为11例（30.6%）和7例（19.4%），介于大肠癌和正常大肠粘膜之间。有趣的是，HsAIRK 1的过表达与大肠癌中p53的积聚显著相关，我们的研究结果表明PLK 1或Aurora A的过表达可能具有致病性、预后和增殖的重要性，因此这些有丝分裂相关基因可能成为大肠癌的新的肿瘤标志物。少