Identificatioin of pancreatic stellate cell subsets associated with malignant behavior of pancreatic cancer cells

与胰腺癌细胞恶性行为相关的胰腺星状细胞亚群的鉴定

• 批准号: 23592013
• 负责人: NABAE Toshinaga
• 金额: $ 3.33万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592013/
• 关键词: 膵癌; 膵星細胞; CD90; ポドプラニン; ＣＤ１０; ＣＤ２７１; ＣＤ９０; 膵臓癌; PSC; phenotype

To identify subsets of pancreatic stellate cells which correlate to the malignant behavior of pancreatic cancer cells, we analysed CD90 and podoplanin strongly expressed in cancer stroma. Analysis of human pancreatic cancer specimen revealed CD90 and podoplanin expression were associated with shorter survival time. Pancreatic stellate cells isolated from fresh pancreatic cancer tissue had heterogeneity in CD90 and pdoplanin expression. The proportion of CD90 positive cells increased in a time-dependent manner after sorted CD90 negative cells had been maintained under culture conditions. Migration and invasion of pancreatic cancer cell lines wereassociated with podoplanin expression in stromal cells and expression of CD10, matrix metalloproteinase (MMP) 2, and MMP3.

为了鉴定与胰腺癌细胞恶性行为相关的胰腺星状细胞亚群，我们分析了在癌间质中强烈表达的CD90和podoplanin。对人胰腺癌标本的分析显示，CD90和podoplanin的表达与较短的生存时间有关。新鲜胰腺癌组织分离的胰腺星状细胞CD90和pdoplanin表达具有异质性。CD90阴性细胞在培养条件下保持分选后，CD90阳性细胞比例呈时间依赖性增加。胰腺癌细胞系的迁移和侵袭与基质细胞中podoplanin的表达以及CD10、基质金属蛋白酶（MMP） 2和MMP3的表达有关。

项目成果

Podoplanin expression in cancer-associated fibroblasts enhances tumor progression of invasive ductal carcinoma of the pancreas.

• DOI: 10.1186/1476-4598-12-168
• 发表时间: 2013-12-20
• 期刊: Molecular cancer
• 影响因子: 37.3
• 作者: Shindo K;Aishima S;Ohuchida K;Fujiwara K;Fujino M;Mizuuchi Y;Hattori M;Mizumoto K;Tanaka M;Oda Y
• 通讯作者: Oda Y

Clinical Significance of Stromal CD90 and alpha-SMA Expression in Pancreatic Cancer

胰腺癌基质 CD90 和 α-SMA 表达的临床意义

• 作者: 阿部俊也;大内田研宙;遠藤翔;肥川和寛;吉田真樹;千々岩芳朗;奥村隆志;田中友晴;佐田政史;堀岡宏平;藤原謙次;森山大樹;宮坂義浩;大塚隆生;高畑俊一;水元一博;田中雅夫;佐田政史;Masafumi Sada
• 通讯作者: Masafumi Sada

The Functional Heterogeneity of Pancreatic Cancer Cells and Surrounding Stromal Cells in Cancer-Stromal Interactions

胰腺癌细胞和周围基质细胞在癌症-基质相互作用中的功能异质性

• 发表时间: 2011
• 作者: Ohuchida K.;et al
• 通讯作者: et al

膵癌間質におけるCD90とα-SMA発現の意義

胰腺癌基质中CD90和α-SMA表达的意义

• 作者: 阿部俊也;大内田研宙;遠藤翔;肥川和寛;吉田真樹;千々岩芳朗;奥村隆志;田中友晴;佐田政史;堀岡宏平;藤原謙次;森山大樹;宮坂義浩;大塚隆生;高畑俊一;水元一博;田中雅夫;佐田政史
• 通讯作者: 佐田政史