Investigation into epigenetic regulatory mechanism by microRNA in carcinogenesis

microRNA在癌变中的表观遗传调控机制研究

• 批准号: 23592015
• 负责人: TAKAMORI Hiroshi
• 金额: $ 3.24万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592015/
• 关键词: IPMN; エピジェネティクス; EZH2; Ki67; p27Kip1; miR-101; carcinogenesis

EZH2 is associated with the accelerated cell proliferation in pancreatic IPMN via the downregulation of p27Kip1 by analyzing surgically resected pancreatic IPMN. On the other hand, Transfection of pre-miR-101 into pancreatic cell line decreased EZH2 expression in mRNA and protein level. Expression of miR-101 was significantly lower in malignant IPMN than benign IPMN. These result shows miR-101 regulate EZH2 expression in pancreatic IPMN.

通过分析手术切除的胰腺 IPMN，EZH2 通过 p27Kip1 的下调与胰腺 IPMN 中细胞增殖的加速相关。另一方面，将 pre-miR-101 转染胰腺细胞系可降低 EZH2 mRNA 和蛋白水平的表达。恶性IPMN中miR-101的表达显着低于良性IPMN。这些结果表明miR-101调节胰腺IPMN中EZH2的表达。

项目成果

Feasibility of Delta-Shaped Anastomoses in Totally Laparoscopic Distal Gastrectomy

三角吻合在全腹腔镜远端胃切除术中的可行性

• DOI: 10.1159/000332850
• 发表时间: 2011
• 期刊: Eur Surg Res
• 影响因子: 1.6
• 作者: Oki E.;et al
• 通讯作者: et al

Carcinogenesis of Intraductal Papillary Mucinous Neoplasm of the Pancreas: Loss of MicroRNA-101 Promotes Overexpression of Histone Methyltransferase EZH2

• DOI: 10.1245/s10434-011-2068-6
• 发表时间: 2012-07-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Nakahara, Osamu;Takamori, Hiroshi;Baba, Hideo
• 通讯作者: Baba, Hideo

Surgical Complications and the Risk Factors of Totally Laparoscopic Distal Gastrectomy

• DOI: 10.1097/sle.0b013e318219a66b
• 发表时间: 2011-06-01
• 期刊: SURGICAL LAPAROSCOPY ENDOSCOPY & PERCUTANEOUS TECHNIQUES
• 影响因子: 1
• 作者: Oki, Eiji;Sakaguchi, Yoshihisa;Maehara, Yoshihiko
• 通讯作者: Maehara, Yoshihiko