Protective effects of bone marrow-derived mononuclear cells in young mice on ischemic brain damage

幼鼠骨髓源性单核细胞对缺血性脑损伤的保护作用

• 批准号: 23592093
• 负责人: HATA Ryuji
• 金额: $ 3.41万
• 依托单位: Fujita Health University (2012-2013)Ehime University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592093/
• 关键词: cerebral ischemia; aging; stem cell; transplantation; 脳虚血; 骨髄単核球; 再生医療; 再生療法; 骨髄幹細胞

Circulating bone marrow-derived endothelial progenitor cells have been reported to beoriginated from bone marrow-derived mononuclear cells (=BMCs) and implicated in homeostasis of the microvasculature. Decreased levels of circulating endothelial progenitor cells, associated with aging correlate with poor clinical outcomes in a range of cardiovascular diseases. Herein, we transplanted BMCs from young mice (=yBMCs) into aged mice before and after cerebral ischemia. Aged mice transplanted with yBMCs before ischemia reduced ischemic brain damage whereas animals transplanted with yBMCs after ischemia did not. Furthermore, aged mice transplanted with yBMCs depleted of CD34 positive cells before ischemia also reduced ischemic brain damage. Although further investigations must be required to elucidate the fundamental mechanism underlying senescence of the brain vasculature, our results shows the possibility that factors other than CD34 have protective effects on ischemic brain damage.

据报道，循环骨髓来源的内皮祖细胞起源于骨髓来源的单核细胞（=BMCs），并参与微血管的稳态。与衰老相关的循环内皮祖细胞水平下降与一系列心血管疾病的不良临床结果相关。在脑缺血前后，我们将年轻小鼠的BMCs （=yBMCs）移植到老年小鼠体内。老龄小鼠缺血前移植ybmc可减轻缺血性脑损伤，而缺血后移植ybmc则无此作用。此外，老龄小鼠在缺血前移植CD34阳性细胞缺失的ybmc也能减轻缺血性脑损伤。虽然还需要进一步的研究来阐明脑血管系统衰老的基本机制，但我们的研究结果表明，除CD34以外的其他因素可能对缺血性脑损伤具有保护作用。

项目成果

Modeling Alzheimer's Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness

• DOI: 10.1016/j.stem.2013.01.009
• 发表时间: 2013-04-04
• 期刊: CELL STEM CELL
• 影响因子: 23.9
• 作者: Kondo, Takayuki;Asai, Masashi;Inoue, Haruhisa
• 通讯作者: Inoue, Haruhisa

Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats.

• DOI: 10.1038/jcbfm.2010.165
• 发表时间: 2011-03
• 期刊: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation

• DOI: 10.1007/s11418-013-0774-z
• 发表时间: 2014-01-01
• 期刊: JOURNAL OF NATURAL MEDICINES
• 影响因子: 3.3
• 作者: Sumiyoshi, Maho;Sakanaka, Masahiro;Kimura, Yoshiyuki
• 通讯作者: Kimura, Yoshiyuki