The effects of bone marrow derived macrophages on ischemic brain damage

骨髓源性巨噬细胞对缺血性脑损伤的影响

• 批准号: 20591688
• 负责人: HATA Ryuji
• 金额: $ 3.08万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591688/
• 关键词: 脳虚血; 自然免疫; TLR4(Toll like receptor 4); マイクログリア; TLR4 (Toll like receptor 4); キメラマウス; TLR4

Cerebral ischemia triggers acute inflammation, which exacerbates primary brain damage. Activation of the innate immune system is an important component of this inflammatory response. Inflammation occurs through the action of proinflammatory cytokines. Production of these cytokines is initiated by signaling through Toll-like receptors (TLRs). Among them, TLR4 recognizes primarily lipopolysaccharide (LPS) and an increasing evidence points that TLR4 plays a crucial role in the concert mechanism of the cerebra ischemia. In this study, we investigated the effect of targeted disruption of TLR4 on the severity of ischemic brain injury. TLR4 ablation significantly decreased infarct size at 1 day after reperfusion. The amelioration of tissue damage in the absence of TLR4 underscores the importance of innate immunity for the manifestation of ischemic brain injury. Furthermore, TLR4 ablation in the bone marrow derived macrophage significantly decreased infarct size. These results indicate that bone barrow derived macrophages are an important modulator of ischemic brain injury.

脑缺血引发急性炎症，从而加重原发性脑损伤。先天免疫系统的激活是这种炎症反应的重要组成部分。炎症通过促炎细胞因子的作用发生。这些细胞因子的产生是通过Toll样受体（TLR）的信号传导启动的。其中，TLR4主要识别脂多糖（LPS），越来越多的证据表明TLR4在脑缺血的协同机制中起着至关重要的作用。在这项研究中，我们研究了靶向破坏TLR4对缺血性脑损伤严重程度的影响。TLR4消融显著减少再灌注后1天的梗死面积。在缺乏TLR4的情况下组织损伤的改善强调了先天免疫对于缺血性脑损伤表现的重要性。此外，TLR4在骨髓来源的巨噬细胞中的消融显著减小了梗死面积。这些结果表明，巴罗源性巨噬细胞是缺血性脑损伤的重要调节剂。

项目成果

Fine structural study of the innervation of muscle spindles in the internal oblique muscle of the abdominal wall in the adult mouse

• DOI: 10.1093/jmicro/dfp058
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF ELECTRON MICROSCOPY
• 作者: Desaki, Junzo;Ezaki, Taichi;Nishida, Naoya
• 通讯作者: Nishida, Naoya

Downregulation of SPARC expression inhibits cell migration and invasion in malignant gliomas

SPARC表达下调抑制恶性胶质瘤细胞迁移和侵袭

• 发表时间: 2009
• 作者: 瀬野 利太

Conditional deletion of Stat3 promotes neurogenesis and inhibits astrogliogenesis in neural stem cells

• DOI: 10.1016/j.bbrc.2010.03.092
• 发表时间: 2010-04-09
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Cao, Fang;Hata, Ryuji;Sakanaka, Masahiro
• 通讯作者: Sakanaka, Masahiro

Retrosigmoid Suprafloccular Transhorizontal Fissure Approach for Resection of Brainstem Cavernous Malformation

• DOI: 10.1227/01.neu.0000369703.67562.bb
• 发表时间: 2010-06-01
• 期刊: NEUROSURGERY
• 影响因子: 4.8
• 作者: Ohue, Shiro;Fukushima, Takanori;Ohnishi, Takanori
• 通讯作者: Ohnishi, Takanori

Evaluation of Intraoperative Brain Shift Using an Ultrasound-Linked Navigation System for Brain Tumor Surgery

• DOI: 10.2176/nmc.50.291
• 发表时间: 2010-04-01
• 期刊: NEUROLOGIA MEDICO-CHIRURGICA
• 影响因子: 1.9
• 作者: Ohue, Shiro;Kumon, Yoshiaki;Ohnishi, Takanori
• 通讯作者: Ohnishi, Takanori