Community effect on muscle differentiation of C2C12 cells

为了研究群落对C2C12成肌的影响，研究了不同细胞密度下培养的细胞的生长动力学和成肌分化。肌源性分化标记物的表达以密度依赖的方式高。在高密度培养中，当细胞与细胞接触明显时，在增殖期后出现接触抑制，并伴随着CDKIp21、肌肉分化标志物MyoD和mygenin等负调控细胞周期标志物的上调。Drebrin蛋白表达也上调。因此，接触抑制可能通过CDKI p21的表达通过细胞间通讯导致生长停滞，并可能促进细胞融合，即细胞分化为肌细胞。在临床环境中，日常运动导致的肌肉力量增强可能为髋关节骨性关节炎患者提供髋托对功能活动能力的重要影响。

To examine the community effect on C2C12 myogenesis growth kinetics and myogenic differentiation were investigated in cells plated at different cell densities. Expression of a myogenic differentiation marker was found to be high in a density-dependent manner. When cell-cell contact was obvious in high-density plating, contact-inhibition was found after the proliferation stage This was accompanied by up-regulation of negative regulating cell cycle markers including CDKIp21 and muscle differentiation markers MyoD and myogenin. Drebrin protein expression was also up-regulated. Thus contact-inhibition may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion, i.e., cell differentiation to myocyte.In the clinical settings, muscle strength augmentation due to daily exercise may provide an essential effect of a hip brace on functional mobility performance in patients with hip osteoarthritis.