Community effect on muscle differentiation of C2C12 cells

群落对 C2C12 细胞肌肉分化的影响

• 批准号: 23592156
• 负责人: WATANABE HIDEOMI
• 金额: $ 3.24万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592156/
• 关键词: 筋分化; C2C12; 細胞密度; 群生効果; 接触抑制; リハビリテーション; 筋力増強; C2C12筋原細胞; community effect; 股関節装具

To examine the community effect on C2C12 myogenesis growth kinetics and myogenic differentiation were investigated in cells plated at different cell densities. Expression of a myogenic differentiation marker was found to be high in a density-dependent manner. When cell-cell contact was obvious in high-density plating, contact-inhibition was found after the proliferation stage This was accompanied by up-regulation of negative regulating cell cycle markers including CDKIp21 and muscle differentiation markers MyoD and myogenin. Drebrin protein expression was also up-regulated. Thus contact-inhibition may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion, i.e., cell differentiation to myocyte.In the clinical settings, muscle strength augmentation due to daily exercise may provide an essential effect of a hip brace on functional mobility performance in patients with hip osteoarthritis.

为了检查对C2 C12肌生成的群体效应，在以不同细胞密度接种的细胞中研究生长动力学和肌生成分化。发现成肌分化标志物的表达以密度依赖性方式高。当高密度平板培养时细胞间接触明显时，细胞增殖阶段后出现接触抑制，并伴随着细胞周期负调控标志物CDKIp 21和肌分化标志物MyoD、myogenin的上调。dreplastin蛋白表达也上调。因此，接触抑制可以通过CDKI p21的表达经由细胞-细胞通讯诱导生长停滞，并且可以负责进行细胞融合，即，在临床环境中，由于日常锻炼而导致的肌肉力量增强可以提供髋支具对髋关节骨关节炎患者的功能性移动性能的重要影响。