Community effect on muscle differentiation of C2C12 cells

以不同细胞密度的C2C12细胞为研究对象，观察群落对C2C12成肌生长动力学和成肌分化的影响。肌源性分化标记物的表达呈高密度依赖性。高密度镀层中细胞-细胞接触明显时，增殖期后出现接触抑制，并伴有CDKIp21和肌肉分化标志物MyoD、myogenin等负调控细胞周期标志物上调。Drebrin蛋白表达也上调。因此，接触抑制可能通过CDKI p21的表达通过细胞间的交流诱导生长停滞，并可能负责细胞融合的进展，即细胞分化为肌细胞。在临床环境中，由于日常锻炼而增强的肌肉力量可能是髋关节支具对髋关节骨关节炎患者功能活动表现的重要影响。

To examine the community effect on C2C12 myogenesis growth kinetics and myogenic differentiation were investigated in cells plated at different cell densities. Expression of a myogenic differentiation marker was found to be high in a density-dependent manner. When cell-cell contact was obvious in high-density plating, contact-inhibition was found after the proliferation stage This was accompanied by up-regulation of negative regulating cell cycle markers including CDKIp21 and muscle differentiation markers MyoD and myogenin. Drebrin protein expression was also up-regulated. Thus contact-inhibition may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion, i.e., cell differentiation to myocyte.In the clinical settings, muscle strength augmentation due to daily exercise may provide an essential effect of a hip brace on functional mobility performance in patients with hip osteoarthritis.