Peripheral Nerve Regeneration across al Biodegradable Artificial Conduit with Vascularity Containing Autogenous Undifferentiated Bone Marrow Stromal Cells in a Canine Model

犬模型中含有自体未分化骨髓基质细胞的可生物降解人工导管的周围神经再生

• 批准号: 23592163
• 负责人: RYOSUKE Kakinoki
• 金额: $ 3.24万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592163/
• 关键词: 末梢神経再生; 人工神経; 骨髄間葉系幹細胞; 血管含有チューブ; 神経チューブ; 血管茎含有; イヌ; 骨髄幹細胞; 血管茎; 末梢神経; 神経再生; Lac-Z; 犬

We created an ulnar vessel-containing tube (70% polylactic acid and 30% polycaprolactone), to which bone marrow-derived mesenchymal stem cells (BMCs) were implanted. The tube was transplanted to bridge a 3cm ulnar nerve defect in canines. Nerve regeneration through the tube was compared with that in a 3cm-long autogenous ulnar nerve segment. Although the rate of nerve regeneration in the tube was slower than that in the nerve segment, the histomorphological analysis of nerve regeneration in the tube was almost more than 80% of that in the autogenous nerve segment 24 weeks after surgery. When BMCs stained with CM dye-I were transplanted into the tube chamber, some CM-dye I-positive cells demonstrated S100 and GFAP markers in the nerve regenerated within the tube. These results indicated that some of the BMCs transplanted into the tube differentiated into Schwann cells, which might have facilitated nerve regeneration in the vessel-containing tubes with BMC transplantation.

我们制作了一个尺侧血管管（70%聚乳酸和30%聚己内酯），将骨髓间充质干细胞（BMCs）植入其中。该管被移植到犬尺神经缺损3cm处。并与3cm长的自体尺神经段进行比较。虽然管内神经再生的速度比神经节段慢，但术后24周管内神经再生的组织形态学分析几乎是自体神经节段的80%以上。将CM- 1染色的bmc细胞移植到管腔中，一些CM- 1染色阳性细胞在管内再生的神经中显示出S100和GFAP标记物。这些结果表明，部分移植到血管内的BMC细胞分化为雪旺细胞，这可能促进了BMC移植后血管内神经的再生。