Ogg1 protects against the catabolic stress-induced downregulation of chondrocyte activity and the apoptosis in OA.

Ogg1 可防止骨关节炎中分解代谢应激诱导的软骨细胞活性下调和细胞凋亡。

• 批准号: 23592236
• 负责人: YUDOH Kazuo
• 金额: $ 3.24万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592236/
• 关键词: 変形性関節症; 核酸損傷; 酸化ストレス; メカニカルストレス; 核酸損傷修復酵素; 軟骨細胞; 核酸修復酵素; DNA酸化損傷; 細胞ストレス応答; 軟骨変性; 活性酸素; DNA修復酵素

It is well known that chondrocytes produce excess amounts of reactive oxygen species (ROS) as well as proinflammatory cytokines and chemokines in response to mechanical and chemical stresses. An oxidized form of guanine, 8-oxo-7,8-dihydroxyguanine (8-oxoguanine), is a major causative lesion for mutagenesis by ROS, because it can cause a stable base pair with adenine or cytosine during DNA replication. 8-Oxoguanine DNA glycosylase (Ogg1) repairs 8-oxoguanine, one of the most abundant DNA adducts caused by oxygen free radicals. We demonstrated that depletion of cellular antioxidant, Ogg1, in osteoarthritic chondrocytes participates in the degeneration of articular cartilage in OA. Our findings suggest that Ogg1 may have a potential to protect against the catabolic factor-induced down-regulation of chondrocyte activity and apoptosis in OA.

众所周知，软骨细胞会产生过量的活性氧（ROS）以及促炎性细胞因子和趋化因子和趋化因子，以响应机械和化学应激。一种氧化形式的鸟嘌呤，8-氧-7,8-二羟基鸟嘌呤（8-氧气）是ROS诱变的主要病变，因为它可以在DNA复制过程中与稳定的碱基对腺苷或细胞儿童稳定。 8-氧气甘氨酸DNA糖基化酶（OGG1）修复8-氧气，这是由氧自由基引起的最丰富的DNA加合物之一。我们证明了骨关节炎软骨细胞中细胞抗氧化剂OGG1的耗竭参与OA关节软骨的退化。我们的发现表明，OGG1可能有潜力防止OA中软骨细胞活性和细胞凋亡的分解代谢因子诱导的下调。

项目成果

Mitochondrial DNA repair enzyme Ogg1 is essential for protection against the downregulation of chondrocyte activity in OA

线粒体 DNA 修复酶 Ogg1 对于防止 OA 软骨细胞活性下调至关重要

• 发表时间: 2013
• 作者: Yoshioka T;Kurokawa MS;Sato T;Nagai K;Iizuka N;Arito M;Takakuwa Y;Nakano H;Ooka S;Suematsu N;Okamoto K;Yudoh K;Nakamura H;Suzuki N;Ozaki S;Kato T.;三幡 輝久;Yudoh K
• 通讯作者: Yudoh K

Layilin, a talin-binding hyaluronan receptor, is expressed in human articular chondrocytes and synoviocytes and is down-regulated by interleukin-1β

• DOI: 10.1007/s10165-012-0686-x
• 发表时间: 2013-05-01
• 期刊: MODERN RHEUMATOLOGY
• 影响因子: 2.2
• 作者: Murata, Minako;Yudoh, Kazuo;Masuko, Kayo
• 通讯作者: Masuko, Kayo

8-oxoguanine DNA glycosylase activity in OA

OA 中 8-氧代鸟嘌呤 DNA 糖基化酶活性

• 发表时间: 2011
• 作者: Yudoh Kazuo;Karasawa Rie.;Yudoh K
• 通讯作者: Yudoh K

Positive feedback loop via astrocytes causes chronic inflammation in virus-associated myelopathy

• DOI: 10.1093/brain/awt183
• 发表时间: 2013-09-01
• 期刊: BRAIN
• 影响因子: 14.5
• 作者: Ando, Hitoshi;Sato, Tomoo;Yamano, Yoshihisa
• 通讯作者: Yamano, Yoshihisa

Mitochondrial DNA repair enzyme Ogg1 is essential for protection against the downregulation of chondrocyte activity in OA.

线粒体 DNA 修复酶 Ogg1 对于防止 OA 软骨细胞活性下调至关重要。

• 发表时间: 2013
• 作者: Yudoh K;Karasawa R;Yui N.
• 通讯作者: Yui N.