New treatment strategy of bladder cancer indexed by centrosome amplification

点击翻译按钮获取中文摘要

The aims of this study were to identify the relevant gene of centrosome amplification (CA) and its regulating microRNA (miR) in bladder cancer, to establish the CA-positive from CA-negative (KK-47) bladder cancer cell line by the gene transfer technique, and to compare the invasive phenotype, or sensitivity of paclitaxel between parent and gene-transferred cell line. To pick-up the relevant genes to CA, array CGH technique was applied and referred the open miR database to find the relevant miR regulating such genes. As a result, hsa-miR-1225-5p and GBAS was selected as miR and relevant gene to control CA, respectively. The GBAS transferred KK-47 cell lined showed significantly higher cell population with CA than parent KK-47 cell lines. Comparison of invasive phenotype, sensitivity of paclitaxel between parent and gene-transferred cell line are under investigation.