The basic research for the novel treatment for under active bladder -focusing on the construction of troponin system in smooth muscle-

膀胱活动不足新疗法的基础研究-聚焦平滑肌肌钙蛋白系统的构建-

• 批准号: 23592371
• 负责人: KAJIOKA Shunichi
• 金额: $ 3.33万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592371/
• 关键词: トロポニン; 平滑筋; 排尿機能; 低活動膀胱; 膀胱排尿筋; 排尿筋; 筋収縮機構; 膀胱平滑筋; トロポニンT

It has been generally accepted that in smooth muscle contraction, MLCK system but not troponin system is dominant. However, we found cardiac troponin T (cTnT) in urinary bladder smooth muscle.The following present study was thus designed in order to clarify troponin subunits in smooth muscle and to explore the possibility for clinical treatment using detrusor smooth muscle. Troponin is composed of 3 subunits (T, I, and C), and categorized into 3 groups (cardiac, slow skeletal muscle, fast skeletal muscle). We examined all troponin subunits and clarified the expression of not only all types of TnT but also slow skeletal muscle type of troponin I in human detrusor. Further, dominant expression of their genes were recognized compared to other smooth muscle organs. The smooth muscle specific protein, calponin and caldesmon were also expressed at protein level suggesting novel contraction system combined with troponin subunits and their protein.

一般认为，在平滑肌收缩中，MLCK系统而非肌钙蛋白系统起主导作用。然而，我们在膀胱平滑肌中发现了心肌肌钙蛋白T （cTnT）。因此，本研究旨在阐明平滑肌中的肌钙蛋白亚基，并探讨利用逼尿肌平滑肌进行临床治疗的可能性。肌钙蛋白由3个亚基（T、I和C）组成，分为3组（心脏、慢骨骼肌、快骨骼肌）。我们检测了所有肌钙蛋白亚基，明确了肌钙蛋白I在人逼尿肌中的表达，不仅是所有类型的TnT，还有慢骨骼肌类型的肌钙蛋白I。此外，与其他平滑肌器官相比，他们的基因的显性表达被识别出来。平滑肌特异性蛋白、钙钙蛋白和钙调素也在蛋白水平上表达，提示肌钙蛋白亚基及其蛋白结合的新型收缩系统。

项目成果

ブタ及びヒト膀胱平滑筋でのトロポニン収縮蛋白による収縮機構の完成を試みる

尝试用肌钙蛋白收缩蛋白完成猪和人膀胱平滑肌的收缩机制

• 作者: 梶岡俊一;Nouval Shahab ,柚木貴和,関成人,内藤誠二
• 通讯作者: Nouval Shahab ,柚木貴和,関成人,内藤誠二

Obstruction enhances rho-kinase pathway and diminishes protein kin-ase C pathway in carbachol-induced calcium sensitization in contraction of α-toxin permeabilized guinea pig detrusor smooth muscle

在α-毒素透化豚鼠逼尿肌平滑肌收缩过程中，卡巴胆碱诱导的钙敏化中，阻塞增强了rho激酶途径并减少了蛋白激酶C途径

• DOI: 10.1002/nau.21193
• 发表时间: 2012
• 期刊: Neurourol Urodyn
• 作者: Shahab N;et.al.
• 通讯作者: et.al.

The effect of cyclic nucleotides on carbachol-induced calcium sensitization in contraction of alpha-toxin permeabilized human and pig detrusor smooth muscle

环核苷酸对卡巴胆碱诱导的α-毒素透化人和猪逼尿肌平滑肌收缩钙敏化的影响

• 发表时间: 2012
• 作者: Kajioka S;Shahab N;Hayashi M;Takahashi R;Seki N;Naito S
• 通讯作者: Naito S

The comparable study of cyclic nucleotides-induced relaxation in Ca^<2+> dependent and independent contraction of a-toxin permeabilized detrusor smooth muscle

环核苷酸诱导α-毒素透化逼尿肌平滑肌Ca^2依赖性和非依赖性收缩舒张的对比研究

• 发表时间: 2012
• 作者: Kajioka S;Shahab N;Hayashi M;Yunoki T;Naito S
• 通讯作者: Naito S

ヒト膀胱排尿平滑筋のトロポニンサブユニットの分子生物学的包括的検討

人膀​​胱逼尿肌平滑肌肌钙蛋白亚基的综合分子生物学研究

• 发表时间: 2012
• 作者: 梶岡俊一;Nouval Shahab;林摩耶;柚木貴和;内藤誠二;梶岡俊一,Nouval Shahab,高橋良輔,内藤誠二
• 通讯作者: 梶岡俊一,Nouval Shahab,高橋良輔,内藤誠二