The investigation into the molecular and electrophysiological properties of overactive bladder and the research for the novel consequent treatment

阐明了猪逼尿肌平滑肌中atp敏感的K+通道的分子和电生理特性（Kajioka等）。[J]。同样在人逼尿肌平滑肌中，成功记录了atp敏感的K+通道的单通道电流，该通道被β-烟酰胺腺嘌呤二核苷酸（βNAD）激活（Kajioka等）。J urrol 2011出版)。Western Blotting和免疫组化染色均未发现豚鼠出口梗阻膀胱中cd4阳性间质细胞或间隙连接明显增多。然而，免疫组化染色显示M2和M3毒蕈碱受体亚型的表达均因阻塞而增加。对于人逼尿肌Ca2+敏化，我们认为M3受体在ROK和PKC通路中起主导作用，而M2受体通过下调cAMP介导ROK通路。

The molecular and electrophysiological properties of ATP-sensitive K+ channel were clarified in pig detrusor smooth muscle (Kajioka et al. J Pharmacol Exp Ther 2008). Also in human detrusor smooth muscle, the single channel current of ATP-sensitive K+channel were successfully recorded and this channel was activated by β-nicotinamide adenine dinucleotide (βNAD) (Kajioka et al. J Urol 2011 in press).Western Blotting and immunohistochemical staining failed to reveal the significant predominant increase of ckit-positive interstitial cell or gap junction in the outlet obstruction bladder of guinea pig. However, immunohistochemical staining demonstrated that the expression of both M2 and M3 muscarinic receptor subtypes were increased by the obstruction. As for Ca2+ sensitization in human detrusor, we concluded that M3 receptor had a predominant role and comparable contribution to ROK and PKC pathways while it M2 receptor mediated ROK pathway via the down regulation of cAMP.