Analysis of spermatogenesis with reduced quality of the genome in degenerate testis of DNA repair-deficient mice

DNA 修复缺陷小鼠退化睾丸基因组质量下降的精子发生分析

• 批准号: 23592379
• 负责人: NAKANE Hironobu
• 金额: $ 3.24万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592379/
• 关键词: DNA修復欠損マウス; 精子形成; 精巣変性状態; ゲノム不安定性; 精巣幹細胞

We have showed that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] provide a valuable model for impaired spermatogenesis as well as for UV-induced skin tumors in XPA patients. Gene expression analysis of each aged Xpa mice testis showed a different expression pattern of DNA repair gene, cell cycle-related gene(checkpoint), apoptosis-related gene compared to control. We tried to check the different expression pattern of these genes by Real-time PCR and immunohistochemistry. We did not detected these difference of these genes by the above methods. However, we found other pathology of vacuole formation in the aged Xpa mice testis. These findings will help us to understand the impaired spermatogenesis in the Xpa mice.

我们已经证明，着色性干皮病A组（Xpa）基因敲除小鼠[Xpa（-/-）小鼠]为XPA患者的精子发生受损以及UV诱导的皮肤肿瘤提供了有价值的模型。基因表达谱分析显示，各年龄组Xpa小鼠睾丸DNA修复基因、细胞周期相关基因（检查点）、凋亡相关基因的表达模式与对照组不同。我们尝试通过Real-time PCR和免疫组化检测这些基因的不同表达模式。用上述方法均未检测到这些基因的差异。然而，我们发现在老年Xpa小鼠睾丸中空泡形成的其他病理。这些发现将有助于我们了解Xpa小鼠精子发生受损的情况。

项目成果

Mitochondria form continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy,Scanning Electron Microscopy for the Life Sciences (Series : Advances in Microscopy and Microanalysis)

线粒体形成连续的细胞内网络结构，通过高分辨率场发射扫描电子显微镜可视化，用于生命科学的扫描电子显微镜（系列：显微镜和微量分析的进展）

• 发表时间: 2013
• 作者: T. Naguro;H. Nakane;and S. Inaga
• 通讯作者: and S. Inaga

Histological analysis of adipose tissues in Xpg null mice

XPg 缺失小鼠脂肪组织的组织学分析

• 发表时间: 2012
• 作者: Hironobu Nakane;Tadahiro Shiomi;Toshio Kameie;Sumire Inaga;Tomonori Naguro
• 通讯作者: Tomonori Naguro

Histological analysis of impaired spermatogenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice

着色性干皮病 A 组基因 (Xpa) 缺陷小鼠精子发生受损的组织学分析

• 发表时间: 2011
• 作者: Hironobu Nakane;Kiyoji Tanaka;Toshio Kameie;Sumire Inaga;Tomonori Naguro
• 通讯作者: Tomonori Naguro

Histological examination of impaired spermatogenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice

着色性干皮病 A 组基因 (Xpa) 缺陷小鼠精子发生受损的组织学检查

• 发表时间: 2013
• 作者: Hironobu Nakane;Kiyoji Tanaka;Toshio Kameie ,Sumire Inaga;Tomonori Naguro;Toshiyuki Kaidoh
• 通讯作者: Toshiyuki Kaidoh

コケイン症候群モデル動物における腎臓の解析

科凯恩综合征模型动物的肾脏分析

• 发表时间: 2013
• 作者: H Nakane ;T Shiomi ;T Kameie;S Inaga;T Naguro ;T Kaidoh;中根裕信
• 通讯作者: 中根裕信