The development of fertility treatment for patients with premature ovarian failure or diminished ovarian reserve by regulating activin action.

第5.5天的小鼠新生鼠卵巢在添加或不添加孕激素的情况下培养48小时。我们发现激活素βA和βB的mRNA表达减少了一半。基于这一事实，我们研究了孕激素持续暴露对小鼠卵巢功能的影响。16周龄小鼠每4天腹腔注射5 mg玉米油或玉米油溶液，每4天1次。注射16次后，将小鼠与雄性小鼠一起饲养。对一只老鼠产下的幼崽总数进行了比较。Dienogest组更明显(P&lt；0.03)。Dienogest组的末次分娩年龄也较高。我们推测Dienogest通过抑制激活素的作用抑制原始卵泡的激活，维持原始卵泡池。这一结果将适用于包括日本在内的发达国家维持卵巢储备和生育能力，这些国家的女性结婚年龄较大。现在我们来研究这种孕激素效应。

Mouse neonatal ovaries of day 5.5 were cultured with or without progestin for 48 hours. We found the Activin beta A and beta B mRNA expression was decreased to half. Based on the fact we researched the effect of continuous progestin exposure to mouse ovarian function. Five mg of dienogest solved in corn oil or corn oil was given by intraperitoneal injection to sixteen week old mice every four days. After sixteen times injection the mice were housed with male mice. The total numbers of pups born from a mouse were compared. It was greater in dienogest group (P < 0.03). The last delivery age was also higher in dienogest group. We speculate that dienogest suppressed primordial follicle activation and maintained the primordial follicle pool through the suppression of activin action. This result would be applicable for the maintenance of ovarian reserve and fertility in the developed country including Japan, where women get married in older age. Now we investigate this progestin effect.