Repair of the damaged trophoblast cells through CD44-mediated signaling

通过 CD44 介导的信号传导修复受损的滋养层细胞

• 批准号: 23592416
• 负责人: SUGIMURA Motoi
• 金额: $ 3.41万
• 依托单位: Hamamatsu University School of Medicine (2012-2013)Juntendo University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592416/
• 关键词: 絨毛細胞; ＣＤ４４; シグナル伝達; 再生; CD44; sFLT1

The function of CD44v3 and heparin/heparan sulphate (HS) signaling was investigated during trophoblast cell migration. We evaluated the effect of heparin/ HS/CD44v3-mediated processes during scratch wound closure in TCL-1 cells. An in vitro scratch wound healing assay showed enhanced migration of trophoblast cells in a dose-dependent manner in the presence of heparin compared with controls. Conversely, an anti-CD44 function blocking antibody and CD44siRNA suppressed the migration of trophoblast cells in the presence of heparin in a similar scratch assay. Both heparin treatment and in vitro scratch wounding induced the phosphorylation of p21-activated kinase 1 (PAK1), while the anti-CD44v3 antibody suppressed the heparin-induced phosphorylation of PAK1. These results indicate that heparin/heparan sulphate/CD44v3-mediated signaling, in the absence of growth factor networks, enhances the direct repair of the damaged trophoblast layer through the migration of trophoblast cells.

CD 44 v3和肝素/硫酸乙酰肝素（HS）信号转导的功能进行了研究在滋养层细胞迁移。我们评估了肝素/ HS/CD 44 v3介导的过程在TCL-1细胞中划痕伤口闭合过程中的作用。体外划痕伤口愈合试验显示，与对照组相比，肝素存在下滋养层细胞的迁移以剂量依赖性方式增强。相反，在类似的划痕试验中，抗CD 44功能阻断抗体和CD 44 siRNA在肝素存在下抑制滋养层细胞的迁移。肝素处理和体外划痕损伤均诱导p21激活激酶1（PAK 1）的磷酸化，而抗CD 44 v3抗体抑制肝素诱导的PAK 1磷酸化。这些结果表明，肝素/硫酸乙酰肝素/CD 44 v3介导的信号传导，在生长因子网络的情况下，通过滋养层细胞的迁移增强受损滋养层的直接修复。

项目成果

Effectiveness of Heparin during Long-Term Tocolysis.

• DOI: 10.1155/2013/650532
• 发表时间: 2013
• 期刊: ISRN obstetrics and gynecology
• 作者: Inagaki T;Makino S;Yorifuji T;Sugimura M;Takeda S
• 通讯作者: Takeda S

Double vertical compression sutures: A novel conservative approach to managing post-partum haemorrhage due to placenta praevia and atonic bleeding.

双垂直加压缝合：一种治疗前置胎盘和失张力出血引起的产后出血的新颖保守方法。

• DOI: 10.1111/j.1479-828x.2012.01422.x
• 发表时间: 2012
• 期刊: Aust N Z J Obstet Gynaecol.
• 作者: Makino S;tanaka T;Yorifuji T;Koshiishi T;Sugimura M;Takeda S.
• 通讯作者: Takeda S.

Heparin/heparan sulfate/CD44-v3 enhances cell migration in term placenta-derived immortalized human trophoblast cells.

肝素/硫酸乙酰肝素/CD44-v3 增强足月胎盘来源的永生化人滋养层细胞的细胞迁移。

• DOI: 10.1095/biolreprod.111.093690
• 发表时间: 2012
• 期刊: Biol Reprod.
• 作者: Suga N. Sugimura M;Kshiishi T;Yorifuji T;Makino S;Takeda S.
• 通讯作者: Takeda S.