Development of the method of selecting the sperm in the ART (Assisted reproductive technology)

开发ART（辅助生殖技术）中的精子选择方法

• 批准号: 23592420
• 负责人: SUGIYAMA Hitoshi
• 金额: $ 3.16万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592420/
• 关键词: 生殖医療; 精子; 走化性; 受精; 生殖補助医療; 走流性; DNA断片化; 卵外皮

In in vitro fertilization using intracytoplasmic sperm injection (ICSI), a single sperm is injected directly into an egg. Although the physiological separation acts in the natural pregnancy, a sperm provided to ICSI is chosen by an embryologist. We customized method of ICSI and some methods for analyses of the sperm DNA fragmentation in mice to perform in our laboratory, and then it was examined whether chemotaxis could use for mammalian sperm separation in this study, but it could not detect the chemotaxis by methods that we used.

在使用卵胞浆内单精子注射(ICSI)的体外受精中，单个精子直接注射到卵子中。虽然生理分离在自然妊娠中起作用，但提供给ICSI的精子是由胚胎学家选择的。我们定制了ICSI方法和一些分析小鼠精子DNA片段化的方法，然后检验了趋化性是否可以用于哺乳动物精子分离，但我们使用的方法不能检测到趋化性。