Otocystic inoculation of adult stem cells using side population method

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There are no fundamental treatments for genetic hearing loss. We set out this experiment to study feasibility of cell therapy in developing inner ear as a first step to develop such a fundamental treatment. Side population (SP) cells which were identified by their unique ability to efflux DNA binding dyes through an ATP-binding cassette transporter. It is reported that SP cells can be isolated from postnatal mouse cochlea and these cells can differentiate cell expressing markers of HCs and supporting cells. We isolated SP cells from postnatal mouse cochlea and implanted SP cells into the mouse otocysts. Nevertheless, it is not possible to evaluate engraftment of SP cell in the developing inner ears accurately because we could not obtain sufficient number of SP cells for injections into the otocysts.