Hair cell regeneration in cochlea utilizing adeno-associated virus vector ending PC3 gene

利用以 PC3 基因结尾的腺相关病毒载体实现耳蜗毛细胞再生

• 批准号: 18591874
• 负责人: MINODA Ryosei
• 金额: $ 2.62万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591874/
• 关键词: inner ear; PC3; Spiral ganglion; Cochlea; 内耳再生; 有毛細胞; 遺伝子治療; ウイルスベクター; 再生; 新生; BTG3

It is reported that PC3 gene has functions to stop cell division of precursor cells of the cerebellar granule cells in the embryonic stage, and facilitate mathl expression in the cerebellar granule cells. Namely, PC3 promotes the differentiation of the cerebellar granule cells. Mathl, which is a key gene for hair cells differentiation in the embryonic cochlea, is originally identified as a transcription factor to promote the differentiation of the cerebellar granule cells. Considering this fact, PC3 may also facilitate hair cell differentiation in cochlea.The purposes of this study are to assess the PC3 expression in the inner ear in normal rat cochlea, and to perform gene transfer of PC3 to cochlea tissue in vitro in order to determine the function of PC3.PC3 was expressed in cochlear in embryonic stage and the neonatal stage in normal rat cochlea. However, PC3 was not detected in hair cell region contrary to our expectation. PC3 was detected in the spiral ganglion. Considering this fact, PC3 does not regulate mathl expression and PC3 are probably related with the development of the spiral ganglion cells. Now, we are working on gene transfer study utilizing electroporation method to clarify this point and to assess the faction of PC3 in spiral ganglion cells.

据报道，PC 3基因在胚胎期具有阻止小脑颗粒细胞前体细胞分裂的功能，并促进小脑颗粒细胞中mathl的表达。即，PC 3促进小脑颗粒细胞的分化。Mathl基因是胚胎耳蜗毛细胞分化的关键基因，最初被认为是促进小脑颗粒细胞分化的转录因子。本研究的目的是检测PC3在正常大鼠耳蜗内耳的表达，并在体外进行PC3基因转染，以确定PC3的功能。然而，与我们的预期相反，在毛细胞区域未检测到PC 3。PC3在螺旋神经节内表达。因此，PC3不调控mathl的表达，可能与螺旋神经节细胞的发育有关。现在，我们正在利用电穿孔方法进行基因转移研究，以阐明这一点，并评估PC3在螺旋神经节细胞中的作用。

项目成果

Preliminary experience with beta-tricalcium phosphate for use in mastoid cavity obliteration after mastoidectomy

β-磷酸三钙用于乳突切除术后乳突腔闭塞的初步经验

• 发表时间: 2007
• 期刊: Otol Neurotol 28
• 作者: 佐野 のぞみ;兵頭 政光;Minoda R
• 通讯作者: Minoda R