Identification of tumor suppressive nucleic acid medicine in maxillary sinus squamous cell carcionma

上颌窦鳞状细胞癌抑癌核酸药物的鉴定

• 批准号: 23592505
• 负责人: HANAZAWA Toyoyuki
• 金额: $ 3.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592505/
• 关键词: microRNA; 上顎洞癌; 癌抑制遺伝子; miR-874; HDAC1; 上顎癌; マイクロRNA; がん; ゲノム

Our recent studies of microRNA (miRNA) expression signature of maxillary sinus squamous cell carcinoma (MSSCC) demonstrated that microRNA-874 (miR-874) was significantly downregulated in cancer tissues, suggesting miR-874 function as a tumor suppressor. Restoration of mature miR-874 significantly inhibited cancer cell proliferation and induced apoptosis. Expression data of miR-874 transfectants and in silico analysis demonstrated that histone deacetylase 1 (HDAC1) was a direct target of miR-874 regulation. Silencing of the HDAC1 gene significantly inhibited cell proliferation and induced G2/M arrest and cell apoptosis in cancer cell. Downregulation of miR-874 was a frequent event in HNSCC. miR-874 acted as a tumor suppressor and directly targeted HDAC1. Recognition of tumor-suppressive miRNA-mediated cancer pathways provides new insights into the potential mechanisms of MSSCC oncogenesis and suggests novel therapeutic strategies for the disease.

我们最近对上颌窦鳞状细胞癌（MSSCC）microRNA（miRNA）表达特征的研究表明，microRNA-874（miR-874）在癌组织中表达显著下调，提示miR-874具有抑癌作用。成熟miR-874的恢复显著抑制癌细胞增殖并诱导凋亡。miR-874转染子的表达数据和计算机模拟分析表明，组蛋白脱乙酰酶1（HDAC 1）是miR-874调节的直接靶点。沉默HDAC 1基因可显著抑制肿瘤细胞的增殖，并诱导G2/M期阻滞和细胞凋亡。miR-874的下调是HNSCC中的常见事件。miR-874作为肿瘤抑制因子，直接靶向HDAC1。对肿瘤抑制性miRNA介导的癌症通路的认识为MSSCC肿瘤发生的潜在机制提供了新的见解，并为该疾病提出了新的治疗策略。

项目成果

癌抑制型microRNA-375が制御する分子ネットワークの解明と頭頸部扁平上皮癌新規治療戦略の開発

阐明抑癌基因microRNA-375调控的分子网络并开发头颈鳞状细胞癌新治疗策略

• 发表时间: 2012
• 作者: 花澤豊行;野畑二次郎;木下崇;吉川直子;鈴木誉;茶薗英明;櫻井大樹;岡本美孝
• 通讯作者: 岡本美孝

Identification of tumor suppressive microRNAs in maxillary sinus squamous cell carcinoma based on microRNA expression signature

基于microRNA表达特征鉴定上颌窦鳞状细胞癌中的抑癌microRNA

• 发表时间: 2011
• 作者: Toyoyuki Hanazawa;Nijiro Nohata;Naoko Kikkawa;Akihiro Katayama;Yasuaki Harabuchi;Yoshitaka Okamoto;Naohiko Seki
• 通讯作者: Naohiko Seki

Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma.

• DOI: 10.1038/bjc.2013.122
• 发表时间: 2013-04-30
• 期刊: British journal of cancer
• 影响因子: 8.8
• 作者: Nohata N;Hanazawa T;Kinoshita T;Inamine A;Kikkawa N;Itesako T;Yoshino H;Enokida H;Nakagawa M;Okamoto Y;Seki N
• 通讯作者: Seki N

Tumor suppressive microRNA-1 and microRNA-133a cluster regulating molecular targets in head and neck squamous cell carcinoma

头颈鳞状细胞癌中肿瘤抑制性 microRNA-1 和 microRNA-133a 簇调节分子靶点

• 发表时间: 2012
• 作者: Nijiro Nohata;Toyoyuki Hanazawa;Takashi Kinoshita;Naoko Kikkawa;Daiju Sakurai;Miki Fuse;Hirofumi Yoshino;Hideki Enokida;Masayuki Nakagawa;Yoshitaka Okamoto;Naohiko Seki
• 通讯作者: Naohiko Seki

Tumor suppressive microRNA-375 regulates lactate dehydrogenase B in maxillary sinus squamous cell carcinoma

• DOI: 10.3892/ijo.2011.1196
• 发表时间: 2012-01-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Kinoshita, Takashi;Nohata, Nijiro;Seki, Naohiko
• 通讯作者: Seki, Naohiko