Analysis of nueroparalytic keratitics using coculture system of neural and corneal epithelial cells

使用神经和角膜上皮细胞共培养系统分析神经麻痹性角膜炎

• 批准号: 23592571
• 负责人: JI-AE Ko
• 金额: $ 3.33万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592571/
• 关键词: 眼細胞生物学

With the use of a coculture system based on a collagen vitrigel membrane, we have investigated the effects of neural cells on human corneal epithelial (HCE) cells. We found that the presence of neural cells promoted the stratification of HCE cells as well as up-regulated expression of the junction protein N-cadherin. These effects of neural cells were mimicked by conditioned medium prepared from differentiating PC12 cells or by neuropeptides (substance P and CGRP) released from trigeminal nerve cells. Furthermore, antagonists of these neuropeptides inhibited the stimulatory effects of trigeminal neurons on the stratification of and N-cadherin expression by HCE cells. Our results thus indicate that neuropeptides released from trigeminal neurons up-regulate N-cadherin expression in and promote the stratification of corneal epithelial cells, and they therefore suggest that such neurons may play an important role in the maintenance of corneal homeostasis and repair of corneal damage.

使用基于胶原玻璃胶膜的共培养系统，我们研究了神经细胞对人角膜上皮（HCE）细胞的影响。我们发现神经细胞的存在促进了HCE细胞的分层以及连接蛋白N-cadherin的表达上调。神经细胞的这些作用由分化的PC 12细胞制备的条件培养基或由三叉神经细胞释放的神经肽（P物质和CGRP）来模拟。此外，这些神经肽的拮抗剂抑制三叉神经元的分层和N-cadherin表达的HCE细胞的刺激作用。因此，我们的研究结果表明，三叉神经神经元释放的神经肽上调N-钙粘蛋白的表达，并促进角膜上皮细胞的分层，因此，他们建议，这样的神经元可能在维持角膜稳态和角膜损伤的修复中发挥重要作用。

项目成果

Matrix metalloproteinase and cytokine expression in Tenon fibroblasts during scar formation after glaucoma filtration or implant surgery in rats

• DOI: 10.1002/cbf.2923
• 发表时间: 2013-08-01
• 期刊: CELL BIOCHEMISTRY AND FUNCTION
• 影响因子: 3.6
• 作者: Nakamura-Shibasaki, Momoko;Ko, Ji-Ae;Kiuchi, Yoshiaki
• 通讯作者: Kiuchi, Yoshiaki

Differential expression of semaphorin 3A and its receptors during mouse retinal development

• DOI: 10.1002/cbf.2833
• 发表时间: 2012-10
• 期刊: Cell Biochemistry and Function
• 影响因子: 3.6
• 作者: Ji-Ae Ko;Y. Mizuno;M. Shibasaki;K. Yamane;T. Chikama;K. Sonoda;Y. Kiuchi

Up-regulation of semaphorin4A expression in retinal pigment epithelial cell by neural cells

神经细胞上调视网膜色素上皮细胞中semaphorin4A的表达

• 发表时间: 2012
• 作者: 生野恭司;浅井智子;矢野晋作;西田幸二;Ko J.-A.,Shibasaki M.,Chikama T.,Yamane K.,Kiuchi Y.
• 通讯作者: Ko J.-A.,Shibasaki M.,Chikama T.,Yamane K.,Kiuchi Y.

Effects of neural cells on the expression of semaphorin4A in cocultured retinal pigment epithelial cells

神经细胞对共培养视网膜色素上皮细胞semaphorin4A表达的影响

• 发表时间: 2011
• 作者: Kimura K;Morita Y;Orita T;Haruta J;Takeji Y;Sonoda KH;Ko J.-A.,Chikama T.,Yamane K.,Kiuchi Y.
• 通讯作者: Ko J.-A.,Chikama T.,Yamane K.,Kiuchi Y.

Neural cells promotes the stratification of corneal epithelial cells by coculture system

神经细胞共培养系统促进角膜上皮细胞分层

• 发表时间: 2011
• 作者: Orita T;Kimura K;Nishida T;Sonoda KH;Hasegawa E;塚原康友;安田 美穂;Ko J.-A.,Chikama T.,Sonoda KH.
• 通讯作者: Ko J.-A.,Chikama T.,Sonoda KH.