Development of intestinal adhesions prophylaxis after emergency surgery by IFN gamma- STAT1 -PAI-1 signal suppression

我们构建了特异性抑制STAT1的SOCS1表达载体和PAI-1 siRNA载体，并利用这些载体在小鼠肠道粘连模型中检测了粘连的预防作用。我们设5组：A：对照组，B: SOCS1组，C: PAI-1 siRNA组，D: SOCS1 + PAI-1 siRNA组，F: HGF（阳性对照）。结果与F组相当，A、B、C三组组织学黏附程度无差异，但D组、E组黏附程度有明显改善。我们发现骨髓、肝脏和肾脏在所有组中都没有副作用。

We constructed SOCS1 expression vector to suppress STAT1 specifically and PAI-1 siRNA vector, and examined the prevention effect of adhesion in mouse intestinal adhesion model using these vectors.We had set the 5 group: A: Control group, B: SOCS1 group, C: PAI-1 siRNA group, D: SOCS1 + PAI-1 siRNA group, F: HGF (positive control). The results were equivalent to that of the F group was no difference in the three groups A, B, and C histology with the degree of adhesion, but adhesion is improved edge in the D group and E group. We found no side effects of bone marrow, liver, to the kidney in all groups.