Molecular biological studies on the effects of fungi on invasion of host cells by periodontalpathogenic bacteria

在这项研究中，我们研究了C。牙龈卟啉单胞菌对人牙龈上皮细胞和牙龈成纤维细胞粘附和侵袭的影响。热死C。白色念珠菌和水溶性甘露糖蛋白-β-葡聚糖复合物。白色念珠菌不增强牙龈卟啉单胞菌的粘附。然而，用热灭活的C.白色念珠菌或水溶性甘露糖蛋白-β-葡聚糖复合物。白色念珠菌显著增强牙龈卟啉单胞菌的侵袭。此外，C. albicans和C. parapsilosis上调人牙龈上皮细胞和牙龈成纤维细胞的半乳糖凝集素-3分泌。这些结果表明，半乳糖凝集素-3的动力学可能参与了牙龈卟啉单胞菌（革兰氏阴性菌）侵袭的增加，因为半乳糖凝集素-3与存在于革兰氏阴性菌外膜中的LPS结合。

In this study, we investigated the effect of C. albicans on the adhesion and invasion of human gingival epithelial cells and gingival fibroblasts by Porphyromonas gingivalis. Heat-killed C. albicans and water-soluble mannoprotein-beta-glucan complex from C. albicans did not enhance P. gingivalis adhesion. However, pretreatment of the cells with heat-killed C. albicans or water-soluble mannoprotein-beta-glucan complex from C. albicans significantly enhanced P. gingivalis invasion. In addition, C. albicans and C. parapsilosis up-regulate galectin-3 secretion by human gingival epithelial cells and gingival fibroblasts. These results suggest that the dynamics of galectin-3 may participate in the increase in invasion of P. gingivalis which is gram negative bacteria because galectin-3 binds to LPS which exists in gram-negative bacteria outer membrane.