Ontogenic analysis of the PACAP receptor, PAC1R, in mouse major salivary glands

小鼠主要唾液腺 PACAP 受体 PAC1R 的个体发生分析

• 批准号: 23592711
• 负责人: NONAKA Naoko
• 金额: $ 3.41万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592711/
• 关键词: PACAP; PACAPレセプター; 唾液腺; 免疫組織学; 発生学; マウス; ドライマウス; 耳下腺; 顎下腺; 舌下腺; 経鼻投与; 免疫染色

We examined the ontogenic expression of PAC1R in mouse salivary glands. Salivary glands at 0, 3, 5, 7, 14 and 21 days postnatal male C57BL/6 mice were used for the immunohistochemical detection of PAC1R. In parotid gland, the acinus was not clearly formed and PAC1R was not detected until 14 days after birth. At 14 days, PAC1R was detected in the striated duct. In sublingual gland, acinus and duct were formed at 0 days after birth. PAC1R was constantly expressed in the striated duct from 0 day postnatal stage. In the case of submandibular gland, granular duct was not clearly identified and PAC1R was localized in striated first detected in striated duct until 21 days after birth. After 21 days, PAC1R was clearly detected at pillar cells in granular duct. Some of cells in the duct showed intense immunoreactions of PAC1R.

我们研究了PAC 1 R在小鼠唾液腺中的个体发育表达。取出生后0、3、5、7、14和21天的雄性C57 BL/6小鼠的唾液腺进行PAC 1 R的免疫组织化学检测。生后14天腮腺腺泡形成不明显，PAC 1 R阳性。14天时，在纹状管中检测到PAC 1 R。舌下腺腺泡和腺管形成于生后0天。PAC 1 R从生后0天开始在纹管中持续表达。在颌下腺的情况下，颗粒管不清楚，PAC 1 R定位于纹状管中，直到出生后21天才首次在纹状管中检测到。21天后，PAC 1 R在颗粒管的柱细胞中被清楚地检测到。导管内部分细胞呈强阳性反应。

项目成果

Nuclei of the human rphe

人类rphe的细胞核

• 发表时间: 2012
• 期刊: Okajimas Folia Anat Jpn
• 作者: Shibata M;Goto N;Goto J;Nonaka N
• 通讯作者: Nonaka N

Ontogenic analysis of the PACAP receptor, PAC1R, in mouse major salivary glands

小鼠主要唾液腺 PACAP 受体 PAC1R 的个体发生分析

• 发表时间: 2014
• 作者: Naoko Nonaka;Masanori Nakamura
• 通讯作者: Masanori Nakamura

マウス唾液腺の発生過程におけるPACAPレセプター局在の免疫組織学的解析

小鼠唾液腺发育过程中 PACAP 受体定位的免疫组织学分析

• 发表时间: 2014
• 作者: 野中直子;中町智哉;塩田清二;中村雅典
• 通讯作者: 中村雅典

マウス唾液腺の発生過程におけるPACAPレセプター局在の解析

小鼠唾液腺发育过程中 PACAP 受体定位分析

• 发表时间: 2013
• 作者: Naoko Nonaka;Masanori Nakamura;玉井利代子;野中直子,中村雅典
• 通讯作者: 野中直子,中村雅典

Kupffer cells support extramedullary erythropoiesis induced by nitrogen-containing bisphosphonate in splenectomized mice

• DOI: 10.1016/j.cellimm.2011.06.025
• 发表时间: 2011-01-01
• 期刊: CELLULAR IMMUNOLOGY
• 影响因子: 4.3
• 作者: Otsuka, Hirotada;Yagi, Hideki;Nakamura, Masanori
• 通讯作者: Nakamura, Masanori