Ontogenic analysis of the PACAP receptor, PAC1R, in mouse major salivary glands

我们检测了PAC1R在小鼠唾液腺中的个体表达。取出生后0、3、5、7、14、21天的雄性C57BL/6小鼠唾液腺进行PAC1R免疫组织化学检测。在腮腺中，腺泡形成不明显，PAC1R在出生后14天才检测到。第14天，纹状管内可见PAC1R。在舌下腺，出生后0天已形成腺泡和导管。从出生后0d起，PAC1R在纹状管中持续表达。颌下腺至出生后21d，颗粒管仍不清楚，PAC1R首先定位于纹状管的纹状区。21d后，颗粒管内的柱状细胞可见明显的PAC1R表达。胆管内部分细胞呈PAC1R免疫反应阳性。

We examined the ontogenic expression of PAC1R in mouse salivary glands. Salivary glands at 0, 3, 5, 7, 14 and 21 days postnatal male C57BL/6 mice were used for the immunohistochemical detection of PAC1R. In parotid gland, the acinus was not clearly formed and PAC1R was not detected until 14 days after birth. At 14 days, PAC1R was detected in the striated duct. In sublingual gland, acinus and duct were formed at 0 days after birth. PAC1R was constantly expressed in the striated duct from 0 day postnatal stage. In the case of submandibular gland, granular duct was not clearly identified and PAC1R was localized in striated first detected in striated duct until 21 days after birth. After 21 days, PAC1R was clearly detected at pillar cells in granular duct. Some of cells in the duct showed intense immunoreactions of PAC1R.