Regeneration of pulp-dentine complex using stem cells derived from oral mucosa or peripheral blood cell in alginic acid hybrid scaffold

在海藻酸混合支架中使用源自口腔粘膜或外周血细胞的干细胞再生牙髓-牙本质复合物

• 批准号: 23592820
• 负责人: YOSHIKAWA Masataka
• 金额: $ 3.16万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592820/
• 关键词: 口腔粘膜由来細胞; nodule形成; アルギン酸; 担体; 骨形成; オステオカルシン; ハイドロキシアパタイト; 口腔粘膜細胞; 骨髄細胞; 歯髄・象牙質複合体; 歯の再生; 幹細胞; In vitro; Nodule形成; 歯髄-象牙質複合体

Oral mucosa-derived cells showed low rate of proliferation in the culture containing growth factors in the medium. However, nodule was formed.In vivo study, bone marrow cells were suspended in alginic acid gel. The gel was filled in pores of HA scaffolds. The scaffolds were implanted in the subcutis of syngeneic rats. However, osteogenesis in the gel was not promoted. Therefore rat bone marrow cells were seeded in alginic acid sponge obtained by heat crosslinking. The sponge with the cells was inserted in the hollow center of cylindrical porous HA scaffold and implanted in rat subcutis for assessment of osteogenesis. As the result, quantity of osteocalcin significantly increased. Moreover, osteogenesis significantly increased quantitatively.In hybrid scaffold composed of HA and formalin treated polyvinyl alcohol sponge coated with laminin, leucine, tryptophan or lysin, the in vivo examination revealed the significant quantitative increase of bone formation by bone marrow cells.

口腔粘膜来源的细胞在培养基中含有生长因子的培养物中表现出低增殖率。在体内研究中，将骨髓细胞悬浮于海藻酸凝胶中。将凝胶填充在HA支架的孔中。将支架植入同系大鼠皮下。然而，凝胶中的成骨没有得到促进。因此，将大鼠骨髓细胞接种在通过热交联获得的海藻酸海绵中。将含有细胞的海绵插入圆柱形多孔HA支架的中空中心，并植入大鼠皮下组织中以评估成骨作用。结果，骨钙素的量显著增加。在由HA和福尔马林处理的聚乙烯醇海绵组成的混合支架中，涂覆层粘连蛋白、亮氨酸、色氨酸或溶素，体内检查显示骨髓细胞成骨的数量显著增加。

项目成果

Effects of amino acids for osteogenesis by bone marrow cells

氨基酸对骨髓细胞成骨的影响

• 发表时间: 2013
• 作者: Yabuuchi T;Yoshikawa M;Hayashi H.
• 通讯作者: Hayashi H.

新規ハイブリッドアルギン酸ナトリウム/リン酸三カルシウムスポンジ状担体の骨髄幹細胞による骨形成能

新型混合海藻酸钠/磷酸三钙海绵状载体的骨髓干细胞成骨能力

• 发表时间: 2011
• 作者: 柿木栄幸;好川正孝;林宏行
• 通讯作者: 林宏行

Osteogenesis by bone marrow cells in novel hybrid alginate/calcium phosphate sponge scaffold

新型混合海藻酸盐/磷酸钙海绵支架中骨髓细胞的成骨作用

• 发表时间: 2011
• 作者: Kakigi H;Yoshikawa M;Hayashi H.
• 通讯作者: Hayashi H.

Hybrid scaffolds composed of leucine-coated sponge and porous hydroxyapatite for hard tissue formation by bone marrow cells

由亮氨酸涂层海绵和多孔羟基磷灰石组成的混合支架，用于骨髓细胞形成硬组织

• 发表时间: 2014
• 作者: Masataka Yoshikawa;Hideyuki Kakigi Takanori Yabuuchi;Hiroyuki Hayashi
• 通讯作者: Hiroyuki Hayashi

骨髄幹細胞による硬組織形成に及ぼすアミノ酸の効果

氨基酸对骨髓干细胞形成硬组织的影响

• 发表时间: 2013
• 作者: 柿木栄幸;好川正孝;辻則正;林宏行
• 通讯作者: 林宏行