Development of sponge scaffold and osteogenic promoting factors in the scaffold for dentine-pulp complex regeneration

牙本质牙髓复合体再生海绵支架及其成骨促进因子的研制

• 批准号: 20592246
• 负责人: YOSHIKAWA Masataka
• 金额: $ 2.91万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592246/
• 关键词: 歯内療法学; ハイドロキシアパタイト; アルギン酸; ゼラチン; ポリビニルアルコール; セルロース; スポンジ担体; 硬組織再生; 骨髄細胞; 組織工学; 骨髄幹細胞; デキストラン; 担体; スポンジ; ヒアルロン酸

The use of porous ceramics is desirable as a scaffold for osteogenesis by undifferentiated mesenchymal stem cells. In the scaffold, an active proliferation and differentiation to hard tissue formation cells was expected for the cells. Therefore an effective factor to differentiate the cells was examined. As a result, it was recognized in hyaluronic acid, laminin and leucine to promote the cell proliferation and differentiation effectively. And it was recognized that the dextran was effective for the differentiation of the undifferentiated mesenchymal cells. The ceramics scaffold received the bone marrow stem cells in the pores. However, it was found that the attachment of the bone marrow stem cells in the sponge with fibrous structure was impossible because of the fibrous structure. Coating of the dextran to the sponge gave width to the fiber, and attachment of bone marrow stem cells would be possible. Oteogenesis in the sponge by bone marrow stem cells was induced in vitro and in vivo.

使用多孔陶瓷作为未分化间充质干细胞成骨的支架是理想的。在支架中，预期细胞活跃增殖并分化为硬组织形成细胞。因此，检测了分化细胞的有效因子。结果表明，透明质酸、层粘连蛋白和亮氨酸能有效促进细胞增殖和分化。葡聚糖对未分化的间充质细胞的分化是有效的。陶瓷支架在孔隙中接受骨髓干细胞。然而，发现由于纤维结构，骨髓干细胞在具有纤维结构的海绵中的附着是不可能的。将葡聚糖涂在海绵上，使纤维变宽，骨髓干细胞的附着将成为可能。在体外和体内诱导骨髓干细胞在海绵中成骨。

项目成果

Nutrition supply for hard tissue formation in hydroxyapatite scaffold in vivo

体内羟基磷灰石支架硬组织形成的营养供应

• 发表时间: 2010
• 作者: Kakigi H;Yoshikawa M;Tsuji N;Hayashi H;Ohgushi H
• 通讯作者: Ohgushi H

Alginic acid gel for bone marrow cell seeding to form bone in porous hydroxyapatite scaffolds.

用于骨髓细胞接种以在多孔羟基磷灰石支架中形成骨的海藻酸凝胶。

• 发表时间: 2010
• 作者: M.Yoshikawa;H.Kakigi;N.Tsuji;H.;Hayashi;H.Ohgushi
• 通讯作者: H.Ohgushi

骨髄細胞の増殖と石灰化物形成促進におよぼすデキストランの影響

右旋糖酐对骨髓细胞增殖及促进钙化形成的作用

• 发表时间: 2008
• 作者: Yoshikawa M;Shimomura Y;Tsuji N;Ohgushi H. Hayashi H;下村容規,好川正孝,辻則正,藪内崇督,林宏行
• 通讯作者: 下村容規,好川正孝,辻則正,藪内崇督,林宏行

スポンジ状担体へのDextranコートが骨髄幹細胞の硬組織形成に及ぼす影響

海绵状载体上葡聚糖涂层对骨髓干细胞硬组织形成的影响

• 发表时间: 2009
• 期刊: 日本再生歯科医学会雑誌 6
• 作者: 寺下正道;北村知昭;寺下正道;下村容規,好川正孝,林宏行
• 通讯作者: 下村容規,好川正孝,林宏行

Effect of leucine coating for bone formation in porous hydroxy apatite Scaffold.

亮氨酸涂层对多孔羟基磷灰石支架中骨形成的影响。

• 发表时间: 2010
• 作者: M.Yoshikawa;N.Tsuji;H.Kakigi;H.;Hayash;H.Ohgushi
• 通讯作者: H.Ohgushi