Development of sponge scaffold and osteogenic promoting factors in the scaffold for dentine-pulp complex regeneration

多孔陶瓷作为未分化间充质干细胞成骨的支架是理想的。在支架中，预期细胞能积极增殖并向硬组织形成细胞分化。因此，研究了细胞分化的有效因子。因此，它在透明质酸、层粘连蛋白和亮氨酸中被识别，可以有效地促进细胞的增殖和分化。右旋糖酐对未分化间充质细胞的分化有明显的促进作用。陶瓷支架在孔中植入了骨髓干细胞。然而，我们发现骨髓干细胞在具有纤维结构的海绵中由于其纤维结构而无法附着。将葡聚糖包覆在海绵上使纤维更宽，骨髓干细胞的附着也就成为可能。在体外和体内诱导骨髓干细胞成骨海绵。

The use of porous ceramics is desirable as a scaffold for osteogenesis by undifferentiated mesenchymal stem cells. In the scaffold, an active proliferation and differentiation to hard tissue formation cells was expected for the cells. Therefore an effective factor to differentiate the cells was examined. As a result, it was recognized in hyaluronic acid, laminin and leucine to promote the cell proliferation and differentiation effectively. And it was recognized that the dextran was effective for the differentiation of the undifferentiated mesenchymal cells. The ceramics scaffold received the bone marrow stem cells in the pores. However, it was found that the attachment of the bone marrow stem cells in the sponge with fibrous structure was impossible because of the fibrous structure. Coating of the dextran to the sponge gave width to the fiber, and attachment of bone marrow stem cells would be possible. Oteogenesis in the sponge by bone marrow stem cells was induced in vitro and in vivo.