Elucidation of the mechanisms underlying drug resistance of oral cancer based on microRNA analysis and applications of the findings to novel diagnostic and therapeutic strategy

基于microRNA分析阐明口腔癌耐药机制并将研究结果应用于新的诊断和治疗策略

• 批准号: 23592967
• 负责人: NAKAYAMA Hideki
• 金额: $ 3.33万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592967/
• 关键词: マイクロRNA; 薬剤耐性; 口腔癌; microRNA; miR-30a; 口腔扁平上皮癌細胞; 5-FU耐性; サイクリンE2

Five-FU-resistant oral squamous cell carcinoma cell lines expressed more miR-30a than the parent cells, thus resulting in increased G1-arrested cells. Luciferase assay revealed that miR-30a suppresses the translation of cyclin E2. MiR-30a targeting significantly increased the sensitivity to 5-FU of OSCC cells with upregulation of cyclin E2 and downregulation of G1-arrested cells. These results suggest that miR-30a could be a useful target for overcoming 5-FU resistance of OSCC.

5 - fu耐药口腔鳞状细胞癌细胞系比亲本细胞表达更多的miR-30a，从而导致g1阻滞细胞增加。荧光素酶检测显示miR-30a抑制细胞周期蛋白E2的翻译。MiR-30a靶向可显著提高OSCC细胞对5-FU的敏感性，上调细胞周期蛋白E2，下调g1阻滞细胞。这些结果表明，miR-30a可能是克服OSCC 5-FU耐药性的有用靶点。

项目成果

The antiapoptotic mechanism underlying drug resistance of oral squamous cell carcinoma

口腔鳞癌耐药的抗凋亡机制

• 发表时间: 2013
• 作者: Nakayama H;Nagata M;Kawahara K;Nakagawa Y;and Shinohara M
• 通讯作者: and Shinohara M

抜歯後に生じた下行性壊死性縦隔炎の1例

拔牙后下行坏死性纵隔炎一例

• 发表时间: 2012
• 期刊: 日本口腔外科学会誌
• 作者: 髙宗康隆;平木昭光;赤崎幸太;吉武義泰;中山秀樹;篠原正徳
• 通讯作者: 篠原正徳

Histopathological Changes in Parotid and Submandibular Glands of Patients Treated with Preoperative Chemoradiation Therapy for Oral Cancer

• DOI: 10.1269/jrr.11115
• 发表时间: 2012-05-01
• 期刊: JOURNAL OF RADIATION RESEARCH
• 影响因子: 2
• 作者: Teshima, Keiko;Murakami, Ryuji;Shinohara, Masanori
• 通讯作者: Shinohara, Masanori

Overexpression of miR-30a confers 5-FU resistance by inhibiting cell proliferation via G1 phase arrest through targeting cyclin E2 in oral squamous cell carcinoma

在口腔鳞状细胞癌中，miR-30a 的过表达通过靶向细胞周期蛋白 E2，通过 G1 期阻滞抑制细胞增殖，从而赋予 5-FU 耐药性

• 发表时间: 2013
• 作者: Kenta Kawahara;Hideki Nakayama;Masashi Nagata;Ryoji Yoshida;Takuya Tanaka;Yoshihiro Nakagawa;Akiyuki Hirosue;Yuichiro Matsuoka;Yoshihiro Yoshitake;Akimitsu Hiraki;and Masanori Shinohara
• 通讯作者: and Masanori Shinohara

Selective inhibition of nuclear factor‐κB by nuclear factor‐κB essential modulator‐binding domain peptide suppresses the metastasis of highly metastatic oral squamous cell carcinoma

• DOI: 10.1111/j.1349-7006.2011.02174.x
• 发表时间: 2012-03
• 期刊: Cancer Science
• 影响因子: 5.7
• 作者: Takuya Tanaka;H. Nakayama;Y. Yoshitake;A. Irie;M. Nagata;K. Kawahara;Y. Takamune;R. Yoshida;Y. Nakagawa;Hidenao Ogi;S. Shinriki;K. Ota;A. Hiraki;T. Ikebe;Y. Nishimura;M. Shinohara