The mechanism of interaction with parathyroid hormone/parathyroid hormone-related protein receptor signals and Wnt/b-catenin in osteo-remodeling tooth migration model

探讨b-连环蛋白作为一种新的与甲状旁腺激素(PTH)/甲状旁腺激素相关蛋白(PTHrP)受体1(PTHR-1)细胞内C末端部分相互作用的新蛋白在成骨细胞中的作用机制及其功能相关性。B-连环蛋白与PTHR-1的细胞膜特异区(584-589)在物理上相互作用并共定位。B-catenin与PTHR-1结合可抑制Gs/cAMP通路，增强Gq/Ca~(2+)通路，但不影响典型的Wnt通路。现在，为了分析体内成骨分化的机制，我们采用了间歇性甲状旁腺素治疗的OsxCre：B-catenin flx小鼠模型，并将对其进行组织学分析。

To investigate the underlying mechanisms of action and functional relevance of b-catenin in osteoblasts, by examining the role of b-catenin as a novel protein that interacts with the intracellular C-terminal portion of the parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor type 1 (PTHR-1). b-catenin physically interacted and co-localized with the cell membrane-specific region of PTHR-1 (584-589). Binding of b-catenin to PTHR-1 caused suppression of the Gs/cAMP pathway and enhancement of the Gq/Ca2+ pathway, without affecting the canonical Wnt pathway. Now, to analyze the mechanism of osteogenic differentiation in vivo, OsxCre:b-catenin flox mice model is treated intermittently with PTH and will be investigate these histological analysis.