Molecular mechanisms of BSP gene transcription on cementblast

BSP基因在成骨细胞上转录的分子机制

• 批准号: 23593050
• 负责人: YAMAUCHI Masato
• 金额: $ 3.24万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593050/
• 关键词: 骨シアロ蛋白質; Ｒｕｎｘ２; ＢＭＰ－２; Runx2; 転写制御; クロマチン; BMP2; TGF-β1; アメロジェニン; ヘルトウィッヒの上皮鞘(HERS)由来細胞

Bone Sialoprotein(BSP) is a tissue-specific marker of cementblast cells which has shown to be transformed from Hertwig's epithlial cells.To elucidate the mechanisms of gene transcription,3 putative osteoblast specific element 2 consensus motif(OSE2-1~OSE2-3) were investigated the ability of Runx2 to interact with cognate cis-acting elements. Forced expression of Runx2 induced 14 fold enhancement of transcription activity observed with the 6 x tandem repeats of OSE2-2 and 3 fold enhancement with that of OSE2-3, whereas 60% significant reduction was observed with that of OSE2-1. Chromatin immunorecipitation analyses revealed Runx2 were significantly higher recruited to OSE2-2 than to other OSE2s in vivo. BMP-2 increased Runx2 binding to these OSE2s and its transcription, whereas obvious reduction of OSE2-2 transcription induced by TGF-beta 1. These data suggested that the coordinated regulation of chromatin on respective OSE2s with Runx2 were modulated by TGF-beta/BMP signals.

骨唾液酸蛋白（BSP）是由Hertwig上皮细胞转化而来的成骨细胞的组织特异性标志物，为了阐明BSP的基因转录机制，我们研究了成骨细胞特异性元件2共有基序（OSE 2 -1~ OSE 2 -3）Runx 2与同源顺式作用元件相互作用的能力。Runx 2的强制表达诱导了用OSE 2 -2的6x串联重复观察到的14倍转录活性增强和用OSE 2 -3观察到的3倍转录活性增强，而用OSE 2 -1观察到60%的显著降低。染色质免疫沉淀分析显示Runx 2在体内被OSE 2 -2比其他OSE 2显著更高地招募。BMP-2增加Runx 2与这些OSE 2的结合及其转录，而TGF-β 1诱导的OSE 2 -2转录明显减少。这些数据表明，染色质对各自的OSE 2与Runx 2的协调调节由TGF-β/BMP信号调节。

项目成果

ADAMTSL6beta protein rescues fibrillin-1 microfibril disorder in a Marfan

ADAMTSL6β 蛋白可挽救马凡氏原纤维蛋白 1 微原纤维疾病

• 发表时间: 2011
• 期刊: The Journal of biological chemistry
• 作者: Saito M;Kurokawa M;Oda M;Oshima M;Tsutsui K;Kosaka K;Nakao K;Ogawa M;Manabe R;Suda N;Ganjargal G;Hada Y;Noguchi T;Teranaka T;Sekiguchi K;Yoneda T;Tsuji T.
• 通讯作者: Tsuji T.

骨シアロ蛋白質(BSP)遺伝子のRunx2による転写調節機構の解明

Runx2阐明骨唾液蛋白（BSP）基因转录调控机制

• 发表时间: 2013
• 作者: Yamaguchi M;Yamada K;Shimizu M;Yoshino T;Kikuta J;Kasai K.;高野知子,小松知子,宮城敦,石井裕美,池田正一;山内雅人
• 通讯作者: 山内雅人

再生歯ユニットの作製と成体顎骨への生着の解析

再生牙单元的制备及成人颌骨植入分析

• 发表时间: 2011
• 作者: Yoshino F;Yoshida A;Wada-Takahashi S;Sugiyama S;Tokutomi F;Maehata Y;Miyamoto C;Komatsu T;Takahashi S-S;Kobayashi K;Lee M-C;永井香絵,福島秀文,竹内靖博,中村仁美,自見英治郎,牧憲司;高橋直子,桜井敦朗,本間宏実,新谷誠康;水野光政
• 通讯作者: 水野光政

Extracellular matrix administration as a potential therapeutic strategy for periodontal ligament regeneration

细胞外基质给药作为牙周膜再生的潜在治疗策略

• 发表时间: 2012
• 期刊: Expert Opin Biol Ther
• 作者: M. Saito;T. Tsuji
• 通讯作者: T. Tsuji

Wnt11 expression in rat dental pulp and promotional effects of Wnt signaling on odontoblast differentiation

• DOI: 10.1111/cga.12011
• 发表时间: 2013-09-01
• 期刊: CONGENITAL ANOMALIES
• 影响因子: 1.3
• 作者: Koizumi, Yu;Kawashima, Nobuyuki;Suda, Hideaki
• 通讯作者: Suda, Hideaki