Decision of a normal range of cytokines in human saliva.

本研究的目的是调查从全身健康状况正常的个体中获得的唾液细胞因子的正常范围，以开发使用唾液的健康检查程序。唾液从全身健康状况良好的个体中获得，并检测唾液16种细胞因子的水平。使用Bio-Bioscience悬浮阵列系统测量（IL-1 α、IL-1 β、IL-5、IL-6、IL-8、IL-10、IL-12（p40）、IL-12（p70）、IL-18、IFN-γ、LIF、M-CSF、TNF、PDGF-bb、TNF-α和VEGF）。所有受试者的8种细胞因子（IL-1 α、IL-1 β、IL-5、IL-8、IL-18、M-CSF、TNF、VEGF）的值均在可测量范围内。此外，在这些细胞因子中，除IL-5外，其他7种细胞因子在男性中的水平均显著高于女性（P<0.05，Mann-Whitney U检验）。

The aim of this study was to investigate the normal range of salivary cytokines obtained from systemically normal individuals for development of health checkup procedure using saliva.The saliva was obtained from individuals who were in a systemically good health conditions and the levels of salivary 16 cytokines (IL-1 alpha, IL-1 beta, IL-5, IL-6, IL-8, IL-10, IL-12(p40), IL-12(p70), IL-18, IFN-gamma, LIF, M-CSF, MIG, PDGF-bb, TNF-alpha and VEGF) were measured using Bio-Plex suspension array system. Eight cytokines (IL-1 alpha, IL-1 beta, IL-5, IL-8, IL-18, M-CSF, MIG, VEGF) were value of a measurable range at all subjects. In addition, among these cytokine, seven cytokines except IL-5 were significantly high level in male than female (p<0.05, Mann-Whitney U test).As a result of this study, the basic information for development of new procedure for health checkup using saliva was obtained.