Mechanism of Micromolar NaF in anti-osteoclastogenesis

微摩尔NaF抗破骨细胞生成机制

• 批准号: 23593110
• 负责人: ARAKAWA Hirohisa
• 金额: $ 3.41万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593110/
• 关键词: 低濃度フッ化物; 骨芽細胞; 破骨細胞; 実験的歯周病モデル; 炎症状態; 破骨細胞分化; 抗菌活性

We report here that Sprague-Dawley rat tissue samples of P. gingivalis ATCC 33277 group with bone resorption visible on micro computed tomography, exhibited a marked increase in cathepsin K, NFATc1, MMP9, and IL-1beta expression compared with those of P. gingivalis + NaF group. Specimens were also confirmed by staining with tartrate-resistant acid phosphatase and by measuring the distance between the cemento-enamel junction and the alveolar bone crest. Moreover, bone marrow cells formed osteoclasts in vitro with exogenous receptor activator of RANKL or M-CSF and micromolar NaF inhibited osteoclast formation. Additionally, cultured bone marrow cells of NaF group expressed higher levels of osteoprotegerin than did control group. These results suggest a model for understanding the pathogenesis of aggressive bone loss in periodontitis and anti-osteoclastogenesis function of micromolar NaF.

在此，我们报道了在显微计算机断层扫描上可见骨吸收的牙龈假单胞菌ATCC 33277组的SD大鼠组织标本中，组织蛋白酶K、NFATc1、MMP9和IL-1β的表达显著高于牙龈假单胞菌+氟化钠组。标本也通过抗酒石酸酸性磷酸酶染色和测量牙釉质-牙釉质交界处与牙槽骨冠之间的距离来确认。此外，骨髓细胞在外源性受体激活剂RANKL或M-CSF的作用下，可在体外形成破骨细胞，而微摩尔NaF则抑制破骨细胞的形成。此外，NaF组培养的骨髓细胞表达的骨保护素水平高于对照组。这些结果为理解牙周炎侵袭性骨丢失的发病机制和微磨牙NaF的抗破骨作用提供了一个模型。

项目成果

Expression of TANGO in oral cancer

TANGO在口腔癌中的表达

• 发表时间: 2012
• 作者: Sasahira T;Bhawal UK;Kurihara M;Ueda N;Yamamoto K;Kirita T;Kuniyasu H
• 通讯作者: Kuniyasu H

口腔扁平上皮癌におけるCXCL14のエピジェネティックス機構を介した遺伝子発現制御

CXCL14表观遗传机制对口腔鳞状细胞癌基因表达的调控

• 发表时间: 2012
• 作者: バワールウジャール;伊藤由美;安孫子宜光
• 通讯作者: 安孫子宜光

Identification by DNA microarray of genes involved in Candida albicans-treated gingival epithelial cells

• DOI: 10.1111/j.1600-0714.2012.01149.x
• 发表时间: 2012-11-01
• 期刊: JOURNAL OF ORAL PATHOLOGY & MEDICINE
• 影响因子: 3.3
• 作者: Ikuta, Tonami;Bhawal, Ujjal K.;Abiko, Yoshimitsu
• 通讯作者: Abiko, Yoshimitsu

Effect of protamine-hydrolysate peptide (DMX) in oral hygiene

鱼精蛋白水解肽 (DMX) 在口腔卫生中的作用

• 发表时间: 2013
• 作者: Bhawal UK;Kuboyama N;Abiko Y
• 通讯作者: Abiko Y

Epigenetic regulation of CXCL14 expression in oral cancer

口腔癌中CXCL14表达的表观遗传调控

• 发表时间: 2013
• 作者: Bhawal UK;Fukuoka CY;Abiko Y
• 通讯作者: Abiko Y