Mechanism of Micromolar NaF in anti-osteoclastogenesis

点击翻译按钮获取中文摘要

We report here that Sprague-Dawley rat tissue samples of P. gingivalis ATCC 33277 group with bone resorption visible on micro computed tomography, exhibited a marked increase in cathepsin K, NFATc1, MMP9, and IL-1beta expression compared with those of P. gingivalis + NaF group. Specimens were also confirmed by staining with tartrate-resistant acid phosphatase and by measuring the distance between the cemento-enamel junction and the alveolar bone crest. Moreover, bone marrow cells formed osteoclasts in vitro with exogenous receptor activator of RANKL or M-CSF and micromolar NaF inhibited osteoclast formation. Additionally, cultured bone marrow cells of NaF group expressed higher levels of osteoprotegerin than did control group. These results suggest a model for understanding the pathogenesis of aggressive bone loss in periodontitis and anti-osteoclastogenesis function of micromolar NaF.