Development of quantitative functional evaluation system for small animals using malti-modal imaging system

本研究开发了一种用于无麻醉多模态成像的小鼠固定装置。固定装置由使用丙烯酸板的固定板和使用50 ml离心管的固定夹制成。用该固定装置在麻醉和非麻醉状态下，用荧光显像（FLI）和正电子发射断层扫描（PET）评价葡萄糖类似物示踪剂在体内的动力学变化，麻醉状态下测定的脑内示踪剂摄取量较非麻醉状态下降低，且FLI的降低率高于PET。其原因可能与脑血流的改变和示踪剂的差异有关。应注意的是，如果在没有麻醉的情况下更换成像设备进行成像，则可能会发生变化。

Mouse fixation device for multi-modal imaging with no anesthesia was developed in this study. Fixation device was made with fixation plate using acrylic board and fixation folder using 50ml centrifuge tube. The changes of kinetics of glucose analogue tracer in in vivo with and without anesthesia were evaluated using fluorescent imaging (FLI) and positron emission tomography (PET) with this fixation device.The uptake of the tracer in the brain measured with anesthesia was decreased in compared with non anesthesia and the decrease rate in FLI was higher than that in PET. It was thought that the reasons of it were the changes of cerebral blood flow and the differences of the tracers. It should be attention that a change may be occurred if the imaging device is changed for the imaging without anesthesia.