Comparative chromosome painting in Brassicaceae for identification of chromosome homeology and elucidation of karyotype evolution

利用染色体特异性BAC重叠群的FISH技术对拟南芥整条染色体进行涂染，并成功建立了比较染色体涂染（CCP）技术，用于拟南芥科近缘种减数分裂期（粗线期）同源染色体的鉴定。基于这些先决条件，打算将CCP应用于芥菜家族的八个部落的约50个分类群，包括拟南芥属的最近亲属。目的是揭示与五个A.拟南芥染色体和染色体重排，发生在进化过程中，并有助于选择的物种之间的基本染色体数目（X=4至10）的变化。CCP首次应用于植物家族将通过染色体计数（新的或确认的）、通过流式细胞术估计基因组大小以及通过FISH鉴定携带染色体的rDNA来补充。与最近的分子系统学数据相比，染色体同源性的数据有望阐明核型进化和系统发育关系内的菊科。

Painting of entire Arabidopsis thaliana chromosomes by FISH with chromosomespecific BAC contigs and comparative chromosome painting (CCP) for identification of homeologous chromosomes in meiotic stages (pachytene) of related Brassicaceae species have been established successfully. Based on these prerequisites it is intended to apply CCP to ~50 taxa of eight tribes of the mustard family including the closest relatives of the Arabidopsis genus. The aim is to reveal chromosomes/chromosome regions homeologous to the five A. thaliana chromosomes and chromosome rearrangements that occurred during evolution and contributed to the variation of the basic chromosome numbers (X=4 to 10) among the selected species. This first application of CCP to a plant family will be supplemented by chromosome counts (new or confirmative), genome size estimation by flow-cytometry, and identification of rDNA bearing chromosomes by FISH. The data on chromosome homeology are expected to elucidate karyotype evolution and phylogenetic relationships within the Brassicaceae in comparison with recent molecular phylogenetic data.