Peromyscus Laboratory Models for Biomedical Research

用于生物医学研究的 Peromyscus 实验室模型

• 批准号: 8512974
• 负责人: Michael Ray Felder
• 金额: $ 11.52万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-08 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8512974
• 关键词: Adolescent; Adult; American; Animal Experimentation; Animal Model; Animals; Area; Ataxia; Babesiosis; Basic Science; Behavioral; Biomedical Research; Bioterrorism; Breeding; Cell division; Chromosome Mapping; Chromosome Painting; Chromosomes; Collaborations; Communicable Diseases; Communities; Cryopreservation; Data; Deer Mouse; Derivation procedure; Detection; Development; Disease; EST Library; Ehrlichiosis; Embryo; Embryo Transfer; Embryonic Development; Environment; Epidemiology; Epilepsy; Expressed Sequence Tags; Freezing; Future; Gene Expression; Generations; Genetic; Genetic Variation; Genome; Genomic Imprinting; Genomics; Goals; Grant; Habitats; Hormonal; Human; Hybrids; Individual; Institutes; Internet; Investigation; Joints; Laboratories; Laboratory Organism; Large-Scale Sequencing; Letters; Life; Lyme Disease; Methods; Microarray Analysis; Modeling; Modification; Movement Disorders; Mus; National Human Genome Research Institute; Paper; Peromyscus; Physiological; Physiological Adaptation; Physiology; Principal Investigator; Production; Publications; Rattus; Research; Research Personnel; Resources; Rodent; Single Nucleotide Polymorphism; Social Behavior; Source; Study models; Syndrome; Technology; Universities; Virus; Work; Xenobiotic Metabolism; base; cost; environmental toxicology; gene discovery; genetic linkage; genetic manipulation; interest; medical schools; microorganism; mortality; mutant; programs; public health relevance; tool

DESCRIPTION (provided by applicant): The Peromyscus Genetic Stock Center (PGSC) provides a reliable source of disease free peromyscines, and is unmatched for the variety of Peromyscus species and mutants available. The PGSC provides animals for a variety of fields, including human infectious disease, behavioral studies, physiological adaptation, and genetic studies. Two developments should further enhance the interest in Peromyscus as an experimental organism. The NHGRI has selected, based on our submission of a white paper, to do a 6X sequence of one species and 2X sequence of 3 other species. This project is just underway at Baylor Medical College. In collaboration with Dr. Glenn (U. Georgia) a request was made to JGI/DOE for construction and sequencing of 50,000 Peromyscus EST clones. The EST clones have been made and quality assessments completed. This complete sequence data should be available within approximately one month. In addition, a first generation linkage map has revealed closer linkage relationships between Peromysus and Rattus than Peromyscus and Mus. Further linkage and chromosome painting analysis has allowed the construction of a linkage map that is chromosome based. These resources will enhance the importance of Peromyscus as a research tool in areas of gene discovery in the area of repetitive movement disorders, juvenile and adult ataxia, and epilepsy, gene expression in disease and environmental toxicology, and mechanisms of genomic imprinting, Peromyscus is a reservoir for two emerging infectious microorganisms. Hanta virus causes a disease with 40 percent mortality and is a potential bioterrorism agent. The causative agent of Lyme disease is also harbored in Peromyscus and nearly 15,000 cases occur annually. PGSC has provided animals for investigators studying these and other diseases such as Ehrlichiosis, babesiosis and plaque. The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use when needed and to decrease costs. Progress has been in obtaining an increase in embryo production using modifications of the hormonal treatment regimes used in mice. Embryos have been successfully frozen and thawed to undergo cell division. Future work will further refine these methods with the goal of re-derivation of living animals from frozen embryos. PUBLIC HEALTH RELEVANCE (provided by applicant): As a species, Peromyscus naturally occurs in diverse habitats offering unique models for studying genetic variation in relation to adaptation to particular environments. Naturally occurring mutants being maintained offer the opportunity to discover genes involved in several mutants of biomedical significance including two movement disorders. Peromyscus in the wild is a reservoir for two emerging infectious microorganisms.

描述（由申请方提供）：Peromyscus Genetic Stock Center（PGSC）提供了无病peromyscines的可靠来源，并且对于可用的多种Peromyscus种属和突变体来说是无与伦比的。PGSC为各种领域提供动物，包括人类传染病，行为研究，生理适应和遗传研究。两个发展应进一步提高Peromyscus作为实验生物的兴趣。根据我们提交的白色论文，NHGRI选择对一个物种进行6倍测序，对另外3个物种进行2倍测序。这个项目正在贝勒医学院进行。与Glenn博士合作，格鲁吉亚）向JGI/DOE提出了构建和测序50，000个Peromyscus EST克隆的请求。EST克隆已经完成，质量评估已经完成。这一完整的序列数据应在大约一个月内提供。此外，第一代连锁图揭示了更密切的连锁关系Peromysus和鼠比Peromysus和Mus。进一步的连锁和染色体彩绘分析，允许构建一个连锁图是基于染色体的。这些资源将增强Peromyscus作为基因发现领域的研究工具在重复性运动障碍，青少年和成人共济失调，癫痫，疾病和环境毒理学中的基因表达以及基因组印记机制领域的重要性，Peromyscus是两种新兴传染性微生物的储存库。汉他病毒引起的疾病死亡率为40%，是一种潜在的生物恐怖主义制剂。莱姆病的病原体也存在于Peromyscus中，每年发生近15，000例病例。PGSC为研究这些疾病和其他疾病（如埃里希体病、巴贝虫病和斑块）的研究人员提供了动物。该项目的研究方面是继续开发胚胎冷冻保存方法和活体动物的再衍生，以保存稀有突变体和种质，供将来需要时使用，并降低成本。在通过修改用于小鼠的激素治疗方案来增加胚胎产量方面取得了进展。胚胎已经成功地被冷冻和解冻以进行细胞分裂。未来的工作将进一步完善这些方法，目标是从冷冻胚胎中重新获得活体动物。 公共卫生相关性（由申请人提供）：作为一个物种，Peromyscus自然存在于不同的栖息地，为研究与适应特定环境有关的遗传变异提供了独特的模型。保持自然发生的突变体提供了机会，发现基因参与几个突变的生物医学意义，包括两个运动障碍。在野外，鹿鼠是两种新出现的传染性微生物的储存库。