Introduction of Caenorhabditis elegans to evaluate and elucidate the anti-senescence effects of food factors and derivatives

引入秀丽隐杆线虫来评估和阐明食物因子及其衍生物的抗衰老作用

• 批准号: 23617017
• 负责人: NISHIKAWA Yoshikazu
• 金额: $ 3.49万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23617017/
• 关键词: 線虫; 老化; 生体防御; 栄養; 抗酸化物質; 乳酸菌; 自然免疫; 機能性食品因子; 食品因子

The nematode, Caenorhabditis elegans, was used to screen lipid-soluble substances, particularly antioxidants, for the lifespan-prolonging effect. Tocotrienols, astaxanthin, sesamin, and the cell wall of bifidobacteria could extend the lifespan of the nematode. These substances enhanced the worm's tolerance to stresses due to heat, UV irradiation, oxidative chemicals, or bacterial infection; these substances could protect the worms from aging-associated weakness of locomotive ability. Each substance gave the different phenotype of tolerance. The mutants which have loss-of-function genes on the signal induction pathways of p38 MAPK or Insulin/IGF for host defense were used to elucidate the mechanism of the anti-senescence effects. Each functional food factor was definitely dependent on these pathways in different manners.

线虫，秀丽隐杆线虫，被用来筛选脂溶性物质，特别是抗氧化剂，延长寿命的效果。生育三烯酚类、虾青素、芝麻素和双歧杆菌细胞壁都能延长线虫的寿命。这些物质增强了蠕虫对热、紫外线照射、氧化化学物质或细菌感染造成的应激的耐受性;这些物质可以保护蠕虫免受与衰老相关的运动能力减弱的影响。每种物质产生不同的耐受表型。利用p38 MAPK或Insulin/IGF信号转导通路功能缺失的突变体来阐明抗衰老作用的机制。每一种功能性食物因子都以不同的方式依赖于这些途径。

项目成果

Increase in Secretory Sphingomyelinase Activity and Specific Ceramides in the Aorta of Apolipoprotein E Knockout Mice during Aging

• DOI: 10.1248/bpb.b13-00180
• 发表时间: 2013-07-01
• 期刊: BIOLOGICAL & PHARMACEUTICAL BULLETIN
• 影响因子: 2
• 作者: Kobayashi, Keiko;Nagata, Eri;Kikuzaki, Hiroe
• 通讯作者: Kikuzaki, Hiroe

Inhibitory activity of Filipendula ulmaria constituents on recombinant human histidine decarboxylase

• DOI: 10.1016/j.foodchem.2012.10.074
• 发表时间: 2013-06-01
• 期刊: FOOD CHEMISTRY
• 影响因子: 8.8
• 作者: Nitta, Yoko;Kikuzaki, Hiroe;Ueno, Hiroshi
• 通讯作者: Ueno, Hiroshi

Identification of a Novel Glycoside, Leptosin, as a Chemical Marker of Manuka Honey

• DOI: 10.1021/jf300068w
• 发表时间: 2012-04-04
• 期刊: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
• 影响因子: 6.1
• 作者: Kato, Yoji;Umeda, Natsuki;Kikuzaki, Hiroe
• 通讯作者: Kikuzaki, Hiroe

Oligosaccharides from agar inhibit murine intestinal inflammation through the induction of heme oxygenase-1 expression

• DOI: 10.1007/s00535-012-0719-4
• 发表时间: 2013-08-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Higashimura, Yasuki;Naito, Yuji;Yoshikawa, Toshikazu
• 通讯作者: Yoshikawa, Toshikazu

1,5-アンヒドログルシトールの物理化学的性質と利用法

1,5-脱水葡萄糖醇的理化性质和用途

• 发表时间: 2013
• 作者: 三宅千悠;太田沙織;小西洋太郎
• 通讯作者: 小西洋太郎