Live imaging of energy supply for synaptic protein transport

突触蛋白运输能量供应的实时成像

• 批准号: 23650169
• 负责人: MOCHIDA Sumiko
• 金额: $ 2.58万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650169/
• 关键词: 分子; 細胞神経科学; ミトコンドリア; 軸索輸送; シナプス; ＡＴＰ; 蛍光蛋白; イメージング; シナプス前終末; 蛋白; イメ－ジング

Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic neuron. Their conduction velocity in the axon and their accumulation in the axon or into the terminal were impaired with dysfunction of a motor adaptor protein.

Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic神经元。它们在轴突中的传导速度及其在轴突或末端中的积聚受到运动适配器蛋白功能障碍的损害。

项目成果

Functional analysis and distribution pattern of synaptic vesicle 2 (SV2) isoforms in superior cervical ganglion neurons

颈上神经节神经元突触小泡2（SV2）亚型的功能分析和分布模式

• 发表时间: 2012
• 作者: Vogl;C.;Mochida;S.;Whalley;B.J.,Tanifuji;S. & Stephens;G.J
• 通讯作者: G.J

Dynamin isoforms decode action potential firing for synaptic vesicles recycling

Dynamin亚型解码突触小泡回收的动作电位放电

• 发表时间: 2012
• 作者: Tanijuji;S.;Mochida;S
• 通讯作者: S

Ca^<2+> channels and Ca^<2+>-binding proteins mediating synaptic transmission of sympathetic neurons

Ca^2通道和Ca^2结合蛋白介导交感神经元突触传递

• 发表时间: 2011
• 作者: Mori;M.;Tanijuji;S.;Mochida;S
• 通讯作者: S

Levetirecetam inhibits synaptic transmission

左乙西坦抑制突触传递

• 作者: Vogl;C.;Whalley;B.J.;Mochida;S.;Stephens;G.
• 通讯作者: G.

蛋白分子レベルでのシナプスの伝達機序

蛋白质分子水平的突触传递机制

• 发表时间: 2011
• 期刊: 自律神経
• 作者: Lakhanpal;G.;Kim;K.;Kato-Hayashi;M.;Hayashi;Y.;Okamoto;K.;持田澄子
• 通讯作者: 持田澄子