Live imaging of energy supply for synaptic protein transport

在发育（培养3周）和成熟（培养6周）的上级颈神经节（SCG）神经元中制备突触接触，我对CFP标记的线粒体、RFP标记的突触囊泡蛋白和GFP标记的CAST活性区蛋白的轴突运输进行了实时成像，将各种动作电位放电模式应用于突触前神经元。它们在轴突中的传导速度和它们在轴突中或进入末端中的积累随着运动适配器蛋白的功能障碍而受损。

Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic neuron. Their conduction velocity in the axon and their accumulation in the axon or into the terminal were impaired with dysfunction of a motor adaptor protein.