Live imaging of energy supply for synaptic protein transport

准备发育（培养3周）和成熟（培养6周）上颈神经节（SCG）神经元的突触接触，我对cfp标记的线粒体、rfp标记的突触体素（一种突触囊泡蛋白）和gfp标记的CAST（一种活性区蛋白）的轴突运输进行了实时成像，并对突触前神经元应用了各种动作电位放电模式。它们在轴突内的传导速度以及它们在轴突或末端的积累因运动接头蛋白的功能障碍而受损。

Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic neuron. Their conduction velocity in the axon and their accumulation in the axon or into the terminal were impaired with dysfunction of a motor adaptor protein.