Functional studies of presynaptic proteins in neurotransmitter release

To examine roles for presynaptic protein-protein interactions in neurotransmitter release，synapses formed between rat superior cervical ganglion neurons in culture were employed.The synaptic protein interaction site of several presynaptic proteins were synthesized and introduced into the presynaptic neurons through a glass patch pipette，while measuring synaptic transmission evoked by presynaptic action potentials.The change in postsynaptic responses，we speculated the function of the proteins as follows.1)Ca I D12+i D1-binding protein(A)synaptotagmin：trigger of synaptic vesicle fusion，promotion of endocytosis.(B)Doc2α-Munc13 interacion：synaptic vesicle translocation to the release site.2)SNARE-associating proteins(A)N-type Ca Ii D12+I D1 channels：involved in synchronous transmite.2，Voltage-dependent enhancement of transmitter release.(B)P/Q-type Ca I D12+i D1 channels：involved in neurotransmitter release.cSnapin：modulation of synaptotagmin-SNARE complex interaction.(D)Syntaphilin：control of SNARE complex assembly by syntaxin-1 clamping.

To examine roles for presynaptic protein-protein interactions in neurotransmitter release, synapses formed between rat superior cervical ganglion neurons in culture were employed. The synaptic protein interaction site of several presynaptic proteins were synthesized and introduced into the presynaptic neurons through a glass patch pipette, while measuring synaptic transmission evoked by presynaptic action potentials. The change in postsynaptic responses, we speculated the function of the proteins as follows.1)CaィイD12+ィエD1-binding protein(A)synaptotagmin : trigger of synaptic vesicle fusion, promotion of endocytosis.(B)Doc2α-Munc 13 interacion : synaptic vesicle translocation to the release site.2)SNARE-associating proteins(A)N-type CaィイD12+ィエD1 channels : involved in synchronous transmitter release, voltage-dependent enhancement of transmitter release.(B)P/Q-type CaィイD12+ィエD1 channels : involved in neurotransmitter release.cSnapin : modulation of synaptotagmin-SNARE complex interaction.(D)Syntaphilin : control of SNARE complex assembly by syntaxin-1 clamping.