Development of screening method to isolate molecules that regulate behaviors of neurotransmitter receptors at synaptic membranes

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SDS-digested freeze-fracture replica labeling electronmiroscopy (SDS-FRL EM) is a technique that enables visualization of proteins, such as neurotransmitter receptors, on synapse membrane with maintenance of space structure. However, the technique in combination with gene transfection has not been established because of the difficulty in recognition of transfected neurons. In this project, we developed a new technique to distinguish transfected neurons on replica membranes by refolding membrane tethered fluorescent proteins that are introduced as markers after SDS digestion.