Targeted gene expression in single neurons by opto-poration in vivo

点击翻译按钮获取中文摘要

We develop a novel method to introduce exogenous genes in individual neurons of interest in the intact brain. By using a mode-locked Ti:Sapphire laser, the neuronal membrane can be perforated to deliver small molecules including fluorescent dye and DNA plasmid. We have confirmed that fluorescent dye molecules could be reliably introduced into individual neurons in the intact mouse brains. After optimization of the technique, it is expected to be able to deliver DNA plasmid and targeted exogenous gene expression in vivo.