Development of anti-metastatic strategy based on analysis of premetastatic niche

癌细胞在其他远端器官中建立转移灶的步骤取决于癌细胞的生存活性和继发病变的微环境。本项目旨在阐明癌细胞与转移前生态位相互作用的分子机制。我们将人乳腺癌细胞注入小鼠心脏，获得脑转移灶，并对小鼠细胞和人细胞mRNA进行RNA-seq分析。该实验使我们能够确定可能在继发性病变中癌细胞定植中起作用的特定基因集。

The step which cancer cells establish their metastatic lesions in other remote organs is dependent on the survival activity of cancer cells and the microenvironment of the secondary lesion. This project was conducted to clarify the molecular mechanism of the interaction between cancer cells and the premetastatic niche. We injected human breast cancer cells into mouse heart and obtained the brain metastasis lesions followed by the RNA-seq analysis of mRNA derived from both mouse cells and human cells. This experiments allowed us to identify the specific gene sets which may play a role in colonization of cancer cells in the secondary lesion.