Development of anti-metastatic strategy based on analysis of premetastatic niche

基于转移前生态位分析的抗转移策略的制定

• 批准号: 23650601
• 负责人: SAYA Hideyuki
• 金额: $ 2.58万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650601/
• 关键词: 癌; 遺伝子; 細胞・組織; 病理学; シグナル伝達

The step which cancer cells establish their metastatic lesions in other remote organs is dependent on the survival activity of cancer cells and the microenvironment of the secondary lesion. This project was conducted to clarify the molecular mechanism of the interaction between cancer cells and the premetastatic niche. We injected human breast cancer cells into mouse heart and obtained the brain metastasis lesions followed by the RNA-seq analysis of mRNA derived from both mouse cells and human cells. This experiments allowed us to identify the specific gene sets which may play a role in colonization of cancer cells in the secondary lesion.

癌细胞在其他远端器官中建立其转移性病变的步骤取决于癌细胞的存活活性和继发性病变的微环境。本项目旨在阐明癌细胞与转移前小生境相互作用的分子机制。我们将人乳腺癌细胞注射到小鼠心脏中并获得脑转移病灶，随后对来自小鼠细胞和人细胞的mRNA进行RNA-seq分析。该实验使我们能够鉴定可能在继发性病变中的癌细胞定植中起作用的特定基因集。

项目成果

Induction of ZEB Proteins by Inactivation of RB Protein Is Key Determinant of Mesenchymal Phenotype of Breast Cancer

• DOI: 10.1074/jbc.m111.313759
• 发表时间: 2012-03-09
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Arima, Yoshimi;Hayashi, Hidemi;Saya, Hideyuki
• 通讯作者: Saya, Hideyuki

Role of CD44 in cancer stem cells and metastasis. Prenary Lecture5

CD44 在癌症干细胞和转移中的作用。

• 发表时间: 2011
• 作者: Ohsawa S;Akai N;Kunimasa K;Igaki T;Saya H
• 通讯作者: Saya H

Role of CD44 in cancer stem cells and metastasis

CD44在癌症干细胞和转移中的作用

• 发表时间: 2011
• 作者: Ohtani M;Hoshii T;Fujii H;Koyasu S;Hirao A;Matsuda S.;Seno M;Saya H
• 通讯作者: Saya H

Loss of p16 expression is associated with the stem cell characteristics of sureface markers and therapeutic resistance in estrogen receptor-negative breast cancer

p16表达缺失与雌激素受体阴性乳腺癌中Surface标记物的干细胞特征和治疗耐药性相关

• 发表时间: 2011
• 期刊: Int J Cancer
• 影响因子: 6.4
• 作者: Arima Y;Naoki Hayashi;Hidemi Hayashi;Mikako Sasaki;Kazuharu Kai;Eiji Sugihara;Eriko Abe;Atsushi Yoshida;Shuji Mikami;Seigo Nakamura;Hideyuki Saya
• 通讯作者: Hideyuki Saya

Loss of p16 expression is associated with the stem cell characteristics of surface markers and therapeutic resistance in estrogen receptor-negative breast cancer

• DOI: 10.1002/ijc.26271
• 发表时间: 2012-06-01
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Arima, Yoshimi;Hayashi, Naoki;Saya, Hideyuki
• 通讯作者: Saya, Hideyuki