Structural and functional studies on Chloroethene sensor kinase and its application for bioremediation
Structural and functional studies on Chloroethene sensor kinase and its application for bioremediation
批准号:
23651063
负责人:
ODAKA Masafumi
金额:
$2.58万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
Chloroethene is one of the most serious pollutants of soil and underground water, and the construction of its detection and removal systems is a very important problem to be solved. In this study, genes of chloroethene sensor kinase were amplified by PCR with a primers set designed based on amino acid sequences of the known chloroethene sensor kinase using a metagenome prepared from a consortium dechlorinating chloroethene to ethene as a template. The obtained sensor kinase was expressed inEscherichia coli and their structures and function were studied.
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Nishimura: Development of bacterial consortia enriched with a novel Dehalococcoides sp. that dechlorinates trichloroethene toethene
Nishimura:开发富含新型 Dehalococcoides sp 的细菌群落。
DOI:
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发表时间:
2012
期刊:
影响因子:
--
作者:
[M. Yohda, M. Kitajima, M. Iwamoto, N.Tamura, J. Shibaski, S. Koike, M]
通讯作者:
M
次世代シーケンサーを用いた Dehalococcoides sp. Strain ATV-1の全ゲノム解析
使用下一代测序仪对 Dehalococcoides sp. 菌株 ATV-1 进行全基因组分析
DOI:
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发表时间:
期刊:
影响因子:
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作者:
[武知 文音, 養王田 正文, 矢木 修身, 塚原 正俊, 照屋 盛実,, 喜久利 育也, 藤森 一浩, 今田 有美, 鼠尾まい子, 佐藤 万仁, 佐藤 友紀, 矢野 修一, 三輪 友希乃, 町田 雅之, 平野 隆]
通讯作者:
平野 隆
Development of Bacterial Consortia Enriched with a Novel Dehalococcoides sp. That Dechlorinates Trichloroethene to Ethene.
富含新型 Dehalococcoides sp 的细菌群落的开发。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[M Yohda, M Kitajima, M Iwamoto, N Tamura, J Shibaski, S Koike, M Nishimura]
通讯作者:
M Nishimura
Dehalococcoides 属細菌由来クロロエテン類センサーキナーゼの発現と機能解析
Dehalococcoides 细菌氯乙烯传感器激酶的表达和功能分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[武知文音, 荒木智, 前田悠貴, 北嶋瑞樹, 山中保明, 野口恵一, 尾高雅文, 養王田正文]
通讯作者:
養王田正文
正文:Dehalococcoides属細菌由来クロロエテン類センサーキナーゼの発現と機能解析
原文:来自 Dehalococcoides 细菌的氯乙烯传感器激酶的表达和功能分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[武知文音, 荒木智, 前田悠貴, 北嶋瑞樹, 山中保明, 野口恵一, 尾高雅文, 養王田]
通讯作者:
養王田
共 9 条
Elucidation of catalytic mechanisms as well as subunit-assembly of nitrile hydratase family enzymes by using advanced structural studies
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批准号:24350082
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.81万
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财政年份:2012
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负责人:ODAKA Masafumi
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依托单位:
Elucidation of mechanisms for catalytic center maturation and catalysis of nitrile hydratase family enzyme
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批准号:21350089
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.31万
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财政年份:2009
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负责人:ODAKA Masafumi
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依托单位:
Molecular mechanism for biogenesis and function of the cobalt reactioncenter of thiocyanate hydrolase
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批准号:19350080
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.73万
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财政年份:2007
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负责人:ODAKA Masafumi
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依托单位:
Function of a novel metal-binding motif having oxidized cysteine residues
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批准号:12440191
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.16万
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财政年份:2000
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负责人:ODAKA Masafumi
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依托单位:
海外基金