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Identification of cell adhesion molecules and growth factors in the serum & the development of new culture medium

血清中细胞粘附分子和生长因子的鉴定

基本信息

批准号：
23651222
负责人：
NAKAMURA Takanori
金额：
$ 2.33万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651222/
关键词：
血清細胞増殖無血清培地 LDL アポトーシス細胞接着増殖制御因子 FBS 培地

项目摘要

By this research, we performed identification of the blood cell cell adherence and the inhibitory growth factors in various animal sera including FBS, and evaluation of proliferation potential value, and obtained the following results.1. The inactivation serum of various mammals has adhesion inhibitory activity over a floating blood cell system cell, and it turned out that the main a dhesion prevention factors are complement C4bp, H factor, and lipoprotein LDL/VLDL. C4bp, and H factor and LDL/VLDL covered the hydrophobic surface of the cultivation flask laboratory dish preferentially in serum protein, and what is checked so that a floating blood cell cell may not paste up nonspecific was conjectured.2. Identification of the blood cell cell-growth inhibition factor in serum was advanced, and one of them is IgM and it found out cytotoxicity strong also against the oxidization LDL which heats LDL (56 ℃ of inactivation conditions of serum, 30 minutes), and arises. On the other hand, inhibitory activity remarkable in IgG was not able to be found out.3. LDL demand nature of Jurkat was strong in the T -lymph cell stock, and on the other hand, Molt-4 had low LDL demand nature and it turned out an insulin, transferrin, and that only selenious acid (ITS) is increased. Moreover, perfect multiplication of Jurkat found low molecular components other than a growth factor or lipid also for the required thing. If IgM is removed for multiplication of Jurkat against an index and the amount of LDL(s) can be controlled, it is possible that development of the alternative culture medium which is equal to FBS is attained.

通过本研究，我们对包括FBS在内的各种动物血清中的血细胞粘附和生长抑制因子进行了鉴定，并对增殖潜能值进行了评价，得到了以下结果.各种哺乳动物的灭活血清对漂浮的血细胞系细胞具有粘附抑制活性，并且证明主要的粘附预防因子是补体C4 bp、H因子和脂蛋白LDL/VLDL。C4 bp，和H因子和LDL/VLDL覆盖的疏水表面的培养瓶实验室皿优先在血清蛋白，和什么是检查，使一个浮动的血细胞细胞可能不会粘贴起来非特异性.对血清中血细胞生长抑制因子进行了鉴定，其中之一是IgM，发现对加热LDL（56 ℃的血清灭活条件，30分钟）而产生的氧化LDL也有很强的细胞毒性。另一方面，在IgG中没有发现显著的抑制活性. Jurkat的LDL需求性质在T淋巴细胞储备中是强的，另一方面，Molt-4具有低LDL需求性质，并且它变成胰岛素、转铁蛋白，并且仅亚硒酸（ITS）增加。此外，完美的倍增Jurkat发现低分子成分以外的生长因子或脂质也为所需的东西。如果去除IgM用于Jurkat针对指数的增殖，并且可以控制LDL的量，则有可能实现等同于FBS的替代培养基的开发。