Creation of minimal artificial virus replication system by totally chemical syntheses

这项工作的目的是构建由合成的短DNA和多肽纳米囊(合成的病毒衣壳)组成的最小人工病毒。主要结果如下：1)成功构建了将M13噬菌体DNA包裹在β-环多肽纳米胶囊中的人工病毒。2)设计了编码β-环多肽的DNA，但无细胞蛋白质合成系统无法获得目的多肽。3)DNA-β-环肽结合物的自组装可构建DNA修饰的人工病毒衣壳。

The aim of this work is construction of minimal artificial virus that consists of synthetic short DNA and peptide nanocapsule (synthetic viral capsid). The important results are as follows: 1) We succeeded in construction of artificial virus that encapsulated M13 phage DNA in the beta-annulus peptide nanocapsule. 2) DNA that encoded beta-annulus peptide was designed, but the objective peptide could not be obtained by cell-free protein synthesis system. 3) DNA-modified artificial viral capsid was able to be constructed by self-assembly of DNA-beta-annulus peptide conjugate.