Electrochemical regulation of nucleic acids hybridization

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I have been trying to immobilize modified DNA carrying disulfide-bridged deoxyribose (ssDNA) and two terpyridine units (terpy2DNA) on carbon and gold electrode, respectively. I expect that I can monitor the hybridization by the change in their electrochemical properties.ssDNA was immobilized on HPOG electrode through stacking interaction of aromatic molecules such as pyrene and perylene onto the HOPG. terpy2DNA was immobilized on Au electrode by using specific affinity of sulfhydryl group, which was modified at the end of terpy2DNA, to Au. Unfortunately, the electrochemical signal of sulfhydryl/disulfide redox pair in ssDNA was not observed. The potential would be out of the measurement window. terpy2DNA formed intramolecular 1:2 complex with some divalent transition metal ions to form [Fe(terpy)2]2+. The redox profile of [Fe(terpy)2]2+/3+ on terpy2DNA was clearly observed on the Au electrode. The hybridization of terpy2DNA was monitored by frequency dependence on SWV measurement.