reconstruction of an external stimulus-dependent replication system

重建外部刺激依赖性复制系统

• 批准号: 23655164
• 负责人: WATANABE Satoru
• 金额: $ 2.58万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655164/
• 关键词: 複製; 合成生物学; DNA; 抗体; 再構成; cis-特異的; 無細胞合成系; 分子進化

Human has a natural gift to select appropriate antibody molecules from huge variety of molecular library, while an engineering technique that reconstructs the selection process against an antigen of interest in a test tube is immature because the variety of the library molecules is a critical and limiting factor of the current technique. I propose a new and challenging idea that breaks the limitation by looking each DNA molecule as a test tube. The feasibility of the idea was experimentally studied and some elementary steps of the idea were shown to be realized.

人类具有从丰富多样的分子文库中选择合适抗体分子的天赋，而在试管中重建针对感兴趣抗原的选择过程的工程技术尚不成熟，因为文库分子的多样性是当前技术的关键和限制因素。我提出了一个具有挑战性的新想法，打破了将每个DNA分子视为试管的限制。实验研究了该思想的可行性，并证明了该思想的一些基本步骤是可以实现的。

项目成果

Flexible and rigid structures in HIV-1 p17 matrix protein monitored by relaxation and amide proton exchange with NMR

通过 NMR 监测松弛和酰胺质子交换监测 HIV-1 p17 基质蛋白的柔性和刚性结构

• 发表时间: 2014
• 期刊: Biochim. Biophys. Acta
• 作者: Ohori;Y.;Okazaki;H.;Watanabe;S.;Tochio;N.;Arai;M.;Kigawa;T.;& Nishimura;C.
• 通讯作者: C.

Flexible and rigid structures in HIV-1 p17 matrix protein monitored by relaxation and amide proton exchange using NMR

使用 NMR 通过弛豫和酰胺质子交换监测 HIV-1 p17 基质蛋白的柔性和刚性结构

• DOI: 10.1016/j.bbapap.2013.12.010
• 发表时间: 2014
• 期刊: Biochimica et Biophysica Acta
• 作者: Ohori;Y.;Okazaki;H.;Watanabe;S.;Tochio;N.;Arai;M.;Kigawa;T.;and Nishimura;C.*
• 通讯作者: C.*

Cell-free synthesis system suitable for disulfide-containing proteins

• DOI: 10.1016/j.bbrc.2012.12.107
• 发表时间: 2013-02-08
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Matsuda, Takayoshi;Watanabe, Satoru;Kigawa, Takanori
• 通讯作者: Kigawa, Takanori