Developmental study for the animal holding facility with fully-enclosed life support system

为研究动物饲养设施（RAHF）准备的最重要的条件包括以下项目：控制空气的成分、温度、湿度和压力，去除空气中的痕量污染物/气味，提供食物和水，废物管理等。本实验室设计的RAHF是在这种条件下自动控制的。第一年（1987年）的一个主题缩小到去除空气中的铵（NH）和CO，这些物质来自动物的尿液和粪便以及食物残渣。本研究所使用的方法是在淋浴室中，NH溶解到闪蒸水中。当控制闪蒸水的PH值和温度时，某些细菌可能会增殖，并可能分解或在NH上生存。水溶性痕量污染物在闪蒸水中溶解。另一种有毒气体CO很容易氧化成CO。然后，有毒物质c 关于我们 空气中的污染物在RAHF中几乎被净化。还通过动物行为和生理数据评估了环境条件。在第二年（1988年），通过遥测系统记录ECG和体温进行生理监测，同时测量作为行为指标的轮旋转次数和作为代谢指标的耗氧量和体重。去年（1989年）进行了长期（3个月）饲养试验。作为结果，闪蒸水中的估计NH浓度与HSO的消耗量良好地对应，直到2个月，但是此后，尽管在空气中以及闪蒸水中保持低水平的NH浓度，HSO的消耗量停止。这一事实意味着NH作为细菌的牲畜可能与尿液和粪便中的NH 4发生平衡。3个月后的气体分析结果表明，RAHF将微量污染物保持在低范围内，生理数据也证明保持了适合仓鼠居住的良好环境条件。少

The most important conditions to be prepared for the research animal holding facility (RAHF) with a fully enclosed life support system are given by following items; to control the composition, temperature, humidity, and pressure of the air, to remove trace contaminants/odors in the air and to supply foods and water, waste management and so forth. The RAHF designed in our laboratory was controlled automatically such conditions. A main theme in the first year (1987) narrowed down to the removal of ammonium (NH ) and CO in the air which originated from animal urine and feces as well as from remnants of foods. A method used in this study was provided a shower chamber in which NH dissolved into flashing water. When PH and temperature of flashing water is controlled, some sort of bacteria may proliferate and may resolve or subsist on NH . Water soluble trace contaminants were resolved in flashing water. Another noxious gas of CO was easily able to oxidize to CO . Then, noxious substance of c … More ontaminants in the air were almost made clean in the RAHF. Environmental conditions were also assessed by animal behavior and physiological data. In the second year (1988) the physiological monitoring by using telemeter system which recorded ECG and body temperature, was carried out in parallel with measurements of the number of wheel rotation as a behavioral index and of oxygen consumption and body weight as a metabolic index. In the last year (1989) the long term (3 months) breeding experiment was conducted. As the results, an estimated NH concentration in the flashing water was well corresponding with the consumption of H SO until 2 months, but thereafter H SO consumption was stopped in spite of keeping the low level of NH concentration in the air and also in the flashing water. This fact means that NH as a livestock of bacteria may balance with occurrence of NH4 from urine and feces. After 3 months results of gas analysis showed the RAHF to keep trace contaminants in low range, and physiological data also testified to maintain a good environmental conditions which were habitable for hamsters. Less