Developmental study for the animal holding facility with fully-enclosed life support system

具有全封闭生命支持系统的研究动物饲养设施（RAHF）需要准备的最重要条件如下：控制空气的成分、温度、湿度和压力，去除空气中的微量污染物/气味，供应食物和水，废物管理等。本实验室设计的RAHF在这种条件下实现了自动控制。第一年（1987年）的主题缩小到去除空气中来自动物尿液和粪便以及食物残渣的铵（NH）和CO。在本研究中使用的方法是提供一个淋浴室，其中NH溶解到闪光水中。当闪蒸水的PH值和温度得到控制时，某些细菌可能会繁殖并在NH上分解或生存。水溶性微量污染物在闪蒸水中被溶解。另一种有毒气体一氧化碳很容易被氧化成一氧化碳。然后，有毒物质c…空气中的更多污染物在RAHF中几乎被净化了。环境条件也通过动物行为和生理数据进行评估。在第二年（1988年），使用遥测系统记录心电图和体温进行生理监测，同时测量车轮转动次数作为行为指标，耗氧量和体重作为代谢指标。最后一年（1989年）进行了为期3个月的长期育种试验。结果表明，在2个月之前，闪蒸水中的NH浓度与氢硫消耗量基本一致，但此后，尽管空气和闪蒸水中的NH浓度保持在较低水平，但氢硫消耗量已停止。这一事实意味着作为细菌家畜的NH4可能与尿液和粪便中的NH4发生平衡。3个月后的气体分析结果表明，RAHF将微量污染物保持在较低的范围内，生理数据也证明了RAHF保持了良好的环境条件，适合仓鼠居住。少

The most important conditions to be prepared for the research animal holding facility (RAHF) with a fully enclosed life support system are given by following items; to control the composition, temperature, humidity, and pressure of the air, to remove trace contaminants/odors in the air and to supply foods and water, waste management and so forth. The RAHF designed in our laboratory was controlled automatically such conditions. A main theme in the first year (1987) narrowed down to the removal of ammonium (NH ) and CO in the air which originated from animal urine and feces as well as from remnants of foods. A method used in this study was provided a shower chamber in which NH dissolved into flashing water. When PH and temperature of flashing water is controlled, some sort of bacteria may proliferate and may resolve or subsist on NH . Water soluble trace contaminants were resolved in flashing water. Another noxious gas of CO was easily able to oxidize to CO . Then, noxious substance of c … More ontaminants in the air were almost made clean in the RAHF. Environmental conditions were also assessed by animal behavior and physiological data. In the second year (1988) the physiological monitoring by using telemeter system which recorded ECG and body temperature, was carried out in parallel with measurements of the number of wheel rotation as a behavioral index and of oxygen consumption and body weight as a metabolic index. In the last year (1989) the long term (3 months) breeding experiment was conducted. As the results, an estimated NH concentration in the flashing water was well corresponding with the consumption of H SO until 2 months, but thereafter H SO consumption was stopped in spite of keeping the low level of NH concentration in the air and also in the flashing water. This fact means that NH as a livestock of bacteria may balance with occurrence of NH4 from urine and feces. After 3 months results of gas analysis showed the RAHF to keep trace contaminants in low range, and physiological data also testified to maintain a good environmental conditions which were habitable for hamsters. Less