Protein interference in mammalian cells and analysis of biological functions of PDI and sEH

在大肠杆菌中发现了蛋白质干扰。藤原教授著。蛋白质干扰是通过结构域-结构域相互作用抑制蛋白质，使整个蛋白质不稳定。在本研究中，蛋白质干扰被用于抑制哺乳动物细胞中的蛋白质。我们首先发现PDI的b′域由a、b、b′、a′和c′组成，它们与a′相互作用。过表达b '而不表达b结构域可显著降低HEK 293细胞的PDI。

Protein interference was found in E. coli. by prof. fujiwara. Protein interference is that suppression of protein by domain-domain interaction which destabilizes whole protein. In this study, protein interference was applied for suppression of protein in mammalian cells. We found first that b’ domain of PDI constituted of a, b, b’ , a’ and c domains interacted with a’. Overexpression of b’ but not b domain significantly reduced the native PDI in HEK 293 cells.