Development of method of inducing cell response by gene expression plasma flow

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To transport of plasma stimuli to cell membrane, we found following results: 1. a primary streamer under water is much suitable to apply charged particles due to its low discharge current. 2. Hydrogen peroxide in the plasma-treated culture medium is not the inactivation factor of cell viability among chemical species when the plasma stimulus is weak though H2O2 is the main in activation factor in the strong plasma stimuli case.3. Analysis using AFM revealed morphologically the cell membrane surface and the plasma effect on the surface shape.