Sterilization Mechanism by Functional Radical Transportation of BiocompatiblePlasma Flow

生物相容性等离子体流的功能性自由基传输的灭菌机制

• 批准号: 17560135
• 负责人: SATO Takehiko
• 金额: $ 2.18万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17560135/
• 关键词: Biocompatible Plasma Flow; Radical Transportation; Sterilization Mechanism; Flow Structure; Atmospheric Pressure; Bacteria; Ultrasensitive Spectroscopy; Visualization; ラジカル; 滅菌

The radical transportation and its sterilization mechanisms in the nonequilibrium plasma flow were studied in this research project for development of innovative plasma sterilization system to protect human environment from infection by pathogenic microorganism. The results of this research project are summarized as follows.Escherichia coli can be sterilized by a low-temperature argon plasma flow at atmospheric pressure and the inactivation of E. coli results from the destructions of the cytoplasmic membrane and the outer membrane under plasma exposure and the destruction of nucleic acids by exposure to ultraviolet radiation from the plasma source. These were clarified by analyzing the surviving cells and the potassium leakage of cytoplasmic material using ICP-MS, and by observing the morphological aspect by SEM. This result was published in the "Applied Physics Letters" and it was selected for the "Virtual Journal of Biological Physics Research". Optical analysis system for very weak emission was successfully developed, which is capable of measuring intensity of 10^<-8> of that in the discharge region. Using this system, the radical flow fields downstream the discharge region was optically investigated.An innovative catheter sterilization system using a nonequilibrium plasma flow was developed. This system is capable of sterilizing anti-UV and anti-heat bacteria within 5 minutes under the condition of low temperature less than 70 degrees Celsius. The sterilization was caused by a radical flow induced by plasma in the tube, which were clarified by analysis systems for the flow fields and radical emissions.Those results were summarized in the 5 academic papers and 7 patents which includes 1 international patent.

本课题研究了非平衡等离子体流中自由基的传输及其杀菌机理，旨在开发新型等离子体杀菌系统，以保护人类环境免受病原微生物的侵害。本研究的主要结果如下：低温氩等离子体能在大气压下对大肠杆菌进行灭菌;大肠杆菌是由于暴露于等离子体下细胞质膜和外膜的破坏以及暴露于来自等离子体源的紫外线辐射下核酸的破坏而产生的。通过使用ICP-MS分析存活细胞和细胞质材料的钾渗漏，并通过SEM观察形态学方面来澄清这些。这一结果发表在《应用物理快报》上，并被选入《生物物理研究虚拟杂志》。研制成功了极微弱辐射的光学分析系统，其测量强度可达<-8>放电区的10倍。利用该系统对放电区下游自由基流场进行了光学研究，并开发了一种新型的非平衡等离子体导管消毒系统。该系统能够在低于70摄氏度的低温条件下，在5分钟内对抗紫外线和耐热细菌进行灭菌。通过流场和自由基辐射分析系统的研究，证实了等离子体在管内产生自由基流，从而实现了杀菌，并在5篇学术论文和7项专利（其中1项国际专利）中进行了总结。

项目成果

Sterilization mechanism for Escherichia coli by plasma flow at atmospheric pressure

• DOI: 10.1063/1.2336594
• 发表时间: 2006-08-14
• 期刊: APPLIED PHYSICS LETTERS
• 影响因子: 4
• 作者: Sato, Takehiko;Miyahara, Takashi;Nakatani, Tatsuyuki
• 通讯作者: Nakatani, Tatsuyuki

Experimental Analysis of Nonequilibrium Plasma Flow at Atmospheric Pressure for Catheter Sterilization

常压非平衡等离子体流导管灭菌实验分析

• 发表时间: 2006
• 期刊: Proceedings of the Third International Conference on Flow Dynamics
• 作者: T.Ushijima;O.Kitoh;牛島達夫;T.Ushijima;午島達夫;落合 史朗;Shiro Ochiai;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya
• 通讯作者: Osamu Furuya

Sterilization of Escherichia Coli by a Low Temperature Coaxial Microwave Plasma Flow at Atmospheric Pressure

大气压下低温同轴微波等离子流对大肠杆菌的灭菌

• 发表时间: 2005
• 期刊: 第18回プラズマ材料科学シンポジウム(SPSM18)アブストラクト集
• 作者: T.Ushijima;O.Kitoh;牛島達夫;T.Ushijima;午島達夫;落合 史朗;Shiro Ochiai;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya;佐藤 岳彦;古屋 修;佐藤 岳彦;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya;Takehiko Sato;Y.Tomita;佐藤 岳彦;Y.Tomita;Takehiko Sato;斉藤 竜也;鴈原 俊太郎;冨田 幸雄;Takehiko Sato;Takehiko Sato;冨田 幸雄;Takehiko Sato
• 通讯作者: Takehiko Sato

Sterilization Efficacy in a Tube by a Nonthermal Plasma Flow at Atmospheric Pressure (in Japanese)

大气压下非热等离子体流在管内的灭菌效果（日语）

• 发表时间: 2006
• 期刊: Proceedings of the 41st Tohoku Branch Regular Meeting of the Japan Society of Mechanical Engineers No.2006-1
• 作者: T.Ushijima;O.Kitoh;牛島達夫;T.Ushijima;午島達夫;落合 史朗;Shiro Ochiai;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya;佐藤 岳彦;古屋 修;佐藤 岳彦;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato
• 通讯作者: Takehiko Sato

Sterilization of Escherichia Coli by a Coaxial Microwave Plasma Flow

同轴微波等离子流对大肠杆菌的灭菌

• 发表时间: 2005
• 期刊: Proceedings of 2005 IEEE Industry Applications Conference 40th IAS Annual Meeting, Hong Kong (CD-ROM)
• 作者: T.Ushijima;O.Kitoh;牛島達夫;T.Ushijima;午島達夫;落合 史朗;Shiro Ochiai;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya;佐藤 岳彦;古屋 修;佐藤 岳彦;Takehiko Sato;Takehiko Sato;Takehiko Sato;Takehiko Sato;Osamu Furuya;Takehiko Sato;Y.Tomita;佐藤 岳彦;Y.Tomita;Takehiko Sato;斉藤 竜也;鴈原 俊太郎;冨田 幸雄;Takehiko Sato;Takehiko Sato
• 通讯作者: Takehiko Sato