Development of highly-efficient process for producing giant unilamellar vesicles (GUV) by controlling microscopic flow field

开发通过控制微观流场高效生产巨型单层囊泡（GUV）的工艺

• 批准号: 23656145
• 负责人: TAKANO Kiyoshi
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23656145/
• 关键词: リポソーム; 振動流; 生体熱工学; マイクロ流動場

We have found that the growth rate of liposomes produced by rehydrating a dried phospholipid layer on a silicone substrate is enhanced by applying the oscillating flow of specific frequency. It has also been found that the initial number of budding liposome from dried phospholipid layer is very sensitive to the electric conductivity of rehydrating solution, although the fluid flow around the initial budding liposome enhances the growing of liposome.

我们发现，通过施加特定频率的振荡流，在硅胶衬底上复水干燥的磷脂层所产生的脂质体的生长速度得到了提高。研究还发现，尽管初始萌发脂体周围的流体流动促进了脂质体的生长，但来自干燥磷脂层的初始萌发脂质体的数量对复水溶液的电导率非常敏感。