The isolation and characterization of cancer associated fibroblasts for clinical therapeutic application.

本研究的目的是分离癌症相关成纤维细胞（CAFs）并对其在胆管癌中的特征进行表征。此外，我们旨在分离正常成纤维细胞，以发现考虑治疗应用的caf与正常成纤维细胞的区别。由于人胆管癌组织难以消化且患者数量有限，我们决定用小鼠模型从慢性炎性肝脏中提取肌成纤维细胞，从正常肝脏中提取正常成纤维细胞。我们进行肝脏灌注并优化消化缓冲液。我们制定了两个群体的细胞分离方案。

The aim of this study is to isolate cancer-associated fibroblasts (CAFs) and characterize them in cholangiocarcinoma. In addition, we aimed to isolate normal fibroblasts to find the difference from CAFs considering therapeutic application. Since the tissue of human cholanigocarcinoma was quite difficult to digest and the number of patients was limited, we decided to get myofibroblasts form chronic inflammatory liver and normal fibroblasts from normal liver using mouse model. We performed liver perfusion and optimized the digestion buffer. We made the protocol of cell separation for both populations.