The isolation and characterization of cancer associated fibroblasts for clinical therapeutic application.

本研究的目的是分离肿瘤相关成纤维细胞(CAF)，并对其在胆管细胞癌中的特性进行研究。此外，我们的目标是分离正常的成纤维细胞，以找出与CAF在治疗应用方面的差异。由于人胆囊癌组织难以消化，患者数量有限，我们决定用小鼠模型分别从慢性炎症性肝组织和正常肝组织中获取肌成纤维细胞和正常肝成纤维细胞。我们进行了肝脏灌注并优化了消化缓冲液。我们制定了两个种群的细胞分离方案。

The aim of this study is to isolate cancer-associated fibroblasts (CAFs) and characterize them in cholangiocarcinoma. In addition, we aimed to isolate normal fibroblasts to find the difference from CAFs considering therapeutic application. Since the tissue of human cholanigocarcinoma was quite difficult to digest and the number of patients was limited, we decided to get myofibroblasts form chronic inflammatory liver and normal fibroblasts from normal liver using mouse model. We performed liver perfusion and optimized the digestion buffer. We made the protocol of cell separation for both populations.