The isolation and characterization of cancer associated fibroblasts for clinical therapeutic application.

用于临床治疗应用的癌症相关成纤维细胞的分离和表征。

• 批准号: 23791549
• 负责人: OKABE Hirohisa
• 金额: $ 2.66万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791549/
• 关键词: CAF（癌関連線維芽細胞）; 胆管癌; 肝星細胞; myofibroblast; 組織消化; 癌関連線維芽細胞; 肝内胆管癌; 線維芽細胞

The aim of this study is to isolate cancer-associated fibroblasts (CAFs) and characterize them in cholangiocarcinoma. In addition, we aimed to isolate normal fibroblasts to find the difference from CAFs considering therapeutic application. Since the tissue of human cholanigocarcinoma was quite difficult to digest and the number of patients was limited, we decided to get myofibroblasts form chronic inflammatory liver and normal fibroblasts from normal liver using mouse model. We performed liver perfusion and optimized the digestion buffer. We made the protocol of cell separation for both populations.

本研究的目的是分离癌症相关成纤维细胞 (CAF) 并表征它们在胆管癌中的特征。此外，考虑到治疗应用，我们的目的是分离正常成纤维细胞，以找出与 CAF 的差异。由于人胆管癌组织相当难以消化且患者数量有限，我们决定利用小鼠模型获取慢性炎症肝脏的肌成纤维细胞和正常肝脏的正常成纤维细胞。我们进行了肝脏灌注并优化了消化缓冲液。我们为这两个群体制定了细胞分离方案。

项目成果

Percentage of future liver remnant volume before portal vein embolization influences the degree of liver regeneration after hepatectomy. J Gastrointest Surg

门静脉栓塞前未来肝脏残余体积的百分比影响肝切除后肝脏再生的程度。

• DOI: 10.1007/s11605-013-2237-y
• 发表时间: 2013
• 期刊: J Gastrointest Surg
• 影响因子: 3.2
• 作者: Okabe H;Beppu T;Nakagawa S;Yoshida M;Hayashi H;Masuda T;Imai K;Mima K;Kuroki H;Nitta H;Hashimoto D;Chikamoto A;Ishiko T;Watanabe M;Yamashita Y;Baba H.
• 通讯作者: Baba H.

Identification of CXCL5/ENA-78 regulating the interaction of cholangiocarcinoma and cancer-associated fibroblasts

鉴定调节胆管癌和癌症相关成纤维细胞相互作用的 CXCL5/ENA-78

• 发表时间: 2012
• 作者: Okabe H;Beppu T;Ueda M;Hayashi H;Masuda T;Mima K;Nakagawa S;Miyake K;Yokoyama N;Baba Y;Takamori H;Ishiko T;Jono H;Shinriki S;Ando Y;Baba H.
• 通讯作者: Baba H.

CD44s signals the acquisition of the mesenchymal phenotype required for anchorage-independent cell survival in hepatocellular carcinoma.

• DOI: 10.1038/bjc.2013.759
• 发表时间: 2014-02-18
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Okabe, H.;Ishimoto, T.;Mima, K.;Nakagawa, S.;Hayashi, H.;Kuroki, H.;Imai, K.;Nitta, H.;Saito, S.;Hashimoto, D.;Chikamoto, A.;Ishiko, T.;Watanabe, M.;Nagano, O.;Beppu, T.;Saya, H.;Baba, H.
• 通讯作者: Baba, H.

β-catenin signaling in murine liver zonation and regeneration: a Wnt-Wnt situation!

• DOI: 10.1002/hep.27082
• 发表时间: 2014-09
• 期刊: HEPATOLOGY
• 影响因子: 13.5
• 作者: Yang, Jing;Mowry, Laura E.;Nejak-Bowen, Kari Nichole;Okabe, Hirohisa;Diegel, Cassandra R.;Lang, Richard A.;Williams, Bart O.;Monga, Satdarshan P.
• 通讯作者: Monga, Satdarshan P.

Side population cells have some stem-like properties in hepatocellular carcinoma

肝细胞癌中的侧群细胞具有一些类似干细胞的特性

• 发表时间: 2011
• 作者: OkabeH;Beppu T;Ishiko T;Chikamoto A;Masuda T;Otao R;Hasita H;Mima K;Takamori H;Baba H.
• 通讯作者: Baba H.