Elucidation of mechanism of the hyperuricemia accompanied by pregnancy induced hypertension (PIH) and multiple pregnancy.

阐明高尿酸血症伴妊娠高血压综合征（PIH）和多胎妊娠的机制。

• 批准号: 23791852
• 负责人: KIMURA Toru
• 金额: $ 2.66万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791852/
• 关键词: 胎盤; 尿酸; 上皮輸送; パラセルラー輸送; 妊娠; 高尿酸血症; 傍細胞輸送; 妊娠高血圧症候群; 多胎妊娠

In this study, we compared maternal serum urate levels with those of umbilical cord blood and investigated urate transport mechanisms in BeWo cells, a trophoblast-derived cell line. There were no significant differences in serum urate levels between maternal blood and umbilical cord blood, suggesting that urate is freely movable at the placenta. RT-PCR and immunohistochemistry showed that urate transporters including OAT4, OAT10, URATv1 and ABCG2 were expressed in the syncytiotrophoblast cells in the placenta as well as BeWo cells. Despite expressing urate transporters, BeWo cells did not take up urate. After confirming the formation of tight junctions of these cells cultured on the transwell, urate transport between upper and lower chambers was measured. Urate moved through BeWo cell monolayers with non-saturation kinetics and this movement was observed even when the cells were incubated at 4 degree, suggesting that urate moves through the paracellular route by simple diffusion.

在这项研究中，我们比较了孕妇血清尿酸盐水平与脐带血和研究尿酸盐转运机制BeWo细胞，滋养层细胞衍生的细胞系。孕妇血和脐带血之间的血清尿酸水平没有显着差异，这表明尿酸盐在胎盘上是自由移动的。RT-PCR和免疫组化结果显示，胎盘合体滋养层细胞和BeWo细胞均表达尿酸盐转运蛋白OAT 4、OAT 10、URATv 1和ABCG 2。尽管表达尿酸盐转运蛋白，BeWo细胞不摄取尿酸盐。在确认在transwell上培养的这些细胞形成紧密连接后，测量上室和下室之间的尿酸盐转运。尿酸盐以非饱和动力学通过BeWo细胞单层移动，即使在4 ℃下孵育细胞时也观察到这种移动，表明尿酸盐通过简单扩散通过细胞旁途径移动。

项目成果

Uric acid crosses the placental barrier through paracellular route

尿酸通过细胞旁途径穿过胎盘屏障

• 发表时间: 2012
• 作者: Toru Kimura;Ichiro Uehara;Shinji Tanigaki;Mitsutoshi Iwashita;Hiroyuki Sakurai
• 通讯作者: Hiroyuki Sakurai

Expression of SLC2A9 isoforms in the kidney and their localization in polarized epithelial cells.

• DOI: 10.1371/journal.pone.0084996
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kimura T;Takahashi M;Yan K;Sakurai H
• 通讯作者: Sakurai H

妊娠時における母体・胎児間の尿酸代謝解析

孕期母婴尿酸代谢分析

• 发表时间: 2013
• 期刊: 発達腎研究会誌
• 作者: 木村徹;上原一朗;谷垣伸治;岩下光利;安西尚彦;櫻井裕之
• 通讯作者: 櫻井裕之

上皮細胞における尿酸のparacellular輸送

上皮细胞中尿酸的细胞旁转运

• 发表时间: 2013
• 作者: 木村徹;塚田愛;市田公美;櫻井裕之
• 通讯作者: 櫻井裕之

Paracellular route is the major urate transport pathway across the blood-placental barrier.

• DOI: 10.14814/phy2.12013
• 发表时间: 2014-05-01
• 期刊: Physiological reports
• 影响因子: 2.5
• 作者: Uehara I;Kimura T;Tanigaki S;Fukutomi T;Sakai K;Shinohara Y;Ichida K;Iwashita M;Sakurai H
• 通讯作者: Sakurai H